Biomarker discovery in serum from patients with carotid atherosclerosis.

Cerebrovascular Diseases Extra
Thomas J DegrabaAnthony F Suffredini

Abstract

Blood-based biomarkers of atherosclerosis have been used to identify patients at high risk for developing stroke. We hypothesized that patients with carotid artery disease would have a distinctive proteomic signature in blood as compared to a healthy control population without carotid artery disease. In order to discover protein biomarkers associated with increased atherosclerotic risk, we used two different strategies to identify biomarkers from patients with clinically defined atherosclerosis who were undergoing endarterectomy for atherosclerotic carotid artery disease. These patients were compared with healthy matched controls. Serum was obtained from patients undergoing endarterectomy (EA; n = 38) and compared to a group of age-matched healthy controls (n = 40). Serum was fractionated using anion exchange chromatography and three different surface-enhanced laser desorption/ionization (SELDI) chip surfaces and then evaluated with mass spectrometry (MS) and two-dimensional difference gel electrophoresis (2D-DIGE). A random forest (RF) analysis of the SELDI-MS protein peak data distinguished these two groups with 69.2% sensitivity and 73.2% specificity. Four unique SELDI peaks (4.2, 4.4, 16.7 and 28 kDa, all p< 0.01) showed th...Continue Reading

Citations

Dec 2, 2015·Journal of Internal Medicine·L PerisicU Hedin
Oct 14, 2014·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Xiang-Pen LiYing Peng
Jun 17, 2018·Scientific Reports·Santosh D BhosaleOlli T Raitakari
Dec 21, 2016·Expert Review of Proteomics·Marika MokouHarald Mischak

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Methods Mentioned

BETA
chip
electrophoresis
affinity capture
chips

Software Mentioned

SPROT
MASCOT
ImageView
Ludesi 2D Interpreter
CiphergenExpress

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