Biomarker potential of ST6GALNAC3 and ZNF660 promoter hypermethylation in prostate cancer tissue and liquid biopsies

Molecular Oncology
Christa HaldrupKarina Dalsgaard Sørensen

Abstract

Current diagnostic and prognostic tools for prostate cancer (PC) are suboptimal, leading to overdiagnosis and overtreatment. Aberrant promoter hypermethylation of specific genes has been suggested as novel candidate biomarkers for PC that may improve diagnosis and prognosis. We here analyzed ST6GALNAC3 and ZNF660 promoter methylation in prostate tissues, and ST6GALNAC3, ZNF660, CCDC181, and HAPLN3 promoter methylation in liquid biopsies. First, using four independent patient sample sets, including a total of 110 nonmalignant (NM) and 705 PC tissue samples, analyzed by methylation-specific qPCR or methylation array, we found that hypermethylation of ST6GALNAC3 and ZNF660 was highly cancer-specific with areas under the curve (AUC) of receiver operating characteristic (ROC) curve analysis of 0.917-0.995 and 0.846-0.903, respectively. Furthermore, ZNF660 hypermethylation was significantly associated with biochemical recurrence in two radical prostatectomy (RP) cohorts of 158 and 392 patients and remained significant also in the subsets of patients with Gleason score ≤7 (univariate Cox regression and log-rank tests, P < 0.05), suggesting that ZNF660 methylation analysis can potentially help to stratify low-/intermediate-grade PCs in...Continue Reading

References

Jan 1, 1985·Statistics in Medicine·J Cuzick
Feb 13, 1999·Nature Genetics·P A Jones, P W Laird
Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman
Apr 1, 2003·The Journal of the American Board of Family Practice·Kishor Mistry, Greg Cable
Jan 7, 2004·Urology·E David Crawford
Jan 7, 2004·Urology·E David Crawford
Jun 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Patrick J BastianWilliam G Nelson
Dec 6, 2005·Nucleic Acids Research·Daniel J WeisenbergerPeter W Laird
Oct 19, 2007·International Journal of Cancer. Journal International Du Cancer·Karina Dalsgaard SørensenNiels Tørring
Aug 19, 2008·The Journal of Urology·Nelly TanJ Stephen Jones
Jan 10, 2009·Clinical Chemistry·Eiji SunamiDave S B Hoon
Feb 28, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sara HeebøllNiels Tørring
May 28, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lorenzo RichiardiFranco Merletti
Sep 17, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Dean A TroyerJoseph W Bigley
Feb 23, 2010·Canadian Urological Association Journal = Journal De L'Association Des Urologues Du Canada·Paul TorenJohn Trachtenberg
Oct 6, 2010·Advances in Genetics·Marta Kulis, Manel Esteller
Feb 24, 2012·Methods in Molecular Biology·Wolfgang GoeringWolfgang A Schulz
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Oct 30, 2012·Folia medica·Ivan V BakardzhievMladena M Mladenova
Jan 17, 2013·British Journal of Cancer·K D SørensenT F Ørntoft
Aug 7, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Christa HaldrupKarina D Sørensen
Apr 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Helle KristensenKarina D Sørensen
Sep 23, 2014·International Journal of Molecular Sciences·Siri H StrandKarina D Sorensen
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Nov 26, 2014·Methods in Molecular Biology·Yanyuan WuJaydutt V Vadgama
Nov 26, 2014·Methods in Molecular Biology·Jong Y Park
Dec 3, 2014·The Journal of Urology·Stacy LoebWilliam J Catalona
Jul 15, 2015·Nature Reviews. Urology·Michael L BluteDavid F Jarrard
Oct 20, 2015·Epigenomics·Pedro Costa-PinheiroCarmen Jerónimo
Nov 1, 2015·Glycobiology·Oliver M T Pearce, Heinz Läubli
Nov 5, 2015·Advances in Experimental Medicine and Biology·J W SalmanM J Roobol
Nov 7, 2015·Cell·UNKNOWN Cancer Genome Atlas Research Network
Nov 13, 2015·Expert Review of Molecular Diagnostics·Jiri PolivkaFilip Janku
Dec 20, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ying-Li LinWen-Ping Li
Dec 25, 2015·BMC Infectious Diseases·Elliot AndersonJeremy Grummet

❮ Previous
Next ❯

Citations

Aug 28, 2020·Frontiers in Oncology·Susana Romero-GarciaAngeles Carlos-Reyes
Aug 28, 2020·Frontiers in Genetics·Beenish RahatJyotdeep Kaur
Oct 24, 2019·Clinical Epigenetics·Maibritt NørgaardKarina D Sørensen
Jul 20, 2020·International Journal of Cancer. Journal International Du Cancer·Isabel HeidrichKlaus Pantel
Oct 21, 2020·Cancers·Dilys LamRuth Pidsley
Nov 30, 2020·Oncogene·Jakob HaldrupKarina D Sorensen
May 16, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maria CoakleyNicholas C Turner
Apr 4, 2021·International Journal of Molecular Sciences·Rossella Indellicato, Marco Trinchera
Jun 3, 2021·International Journal of Molecular Sciences·Cecilia Fernández-PonceElkin Navarro Quiroz
Jul 3, 2021·Cancers·Cora Palanca-BallesterAlfonso Calvo
Nov 14, 2021·Molecular Diagnosis & Therapy·Susana Olmedillas-LópezDamián García-Olmo

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
biopsies
biopsy
blood draw
RNAseq

Software Mentioned

SDS
HTSeq
Tuxedo Suite
Primer3
stata
qMSP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.