Biomass Accumulation and Cell Wall Structure of Rice Plants Overexpressing a Dirigent-Jacalin of Sugarcane (ShDJ ) Under Varying Conditions of Water Availability

Frontiers in Plant Science
Larissa Mara AndradeSilvana Creste

Abstract

A sugarcane gene encoding a dirigent-jacalin, ShDJ, was induced under drought stress. To elucidate its biological function, we integrated a ShDJ-overexpression construction into the rice Nipponbare genome via Agrobacterium-mediated transformation. Two transgenic lines with a single copy gene in T0 were selected and evaluated in both the T1 and T4 generations. Transgenic lines had drastically improved survival rate under water deficit conditions, at rates close to 100%, while WT did not survive. Besides, transgenic lines had improved biomass production and higher tillering under water deficit conditions compared with WT plants. Reduced pectin and hemicellulose contents were observed in transgenic lines compared with wild-type plants under both well-watered and water deficit conditions, whereas cellulose content was unchanged in line #17 and reduced in line #29 under conditions of low water availability. Changes in lignin content under water deficit were only observed in line #17. However, improvements in saccharification were found in both transgenic lines along with changes in the expression of OsNTS1/2 and OsMYB58/63 secondary cell wall biosynthesis genes. ShDJ-overexpression up-regulated the expression of the OsbZIP23, OsGRAS...Continue Reading

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Citations

Mar 9, 2021·Frontiers in Plant Science·Maria Juliana Calderan-RodriguesCamila Caldana

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Methods Mentioned

BETA
transgenic
RNA-seq
electrophoresis
phosphotransferase
PCR
genetic modification

Software Mentioned

MEGA6
ClustalW
Basic Local Alignments Search Tools
ExPASy compute pI / Mw
blast2
BLAST
Mega
REST©
tblastn
SAS

Related Concepts