Biomaterial characterization of off-the-shelf decellularized porcine pericardial tissue for use in prosthetic valvular applications.

Journal of Tissue Engineering and Regenerative Medicine
Joshua A ChoeMelissa D Young

Abstract

Fixed pericardial tissue is commonly used for commercially available xenograft valve implants, and has proven durability, but lacks the capability to remodel and grow. Decellularized porcine pericardial tissue has the promise to outperform fixed tissue and remodel, but the decellularization process has been shown to damage the collagen structure and reduce mechanical integrity of the tissue. Therefore, a comparison of uniaxial tensile properties was performed on decellularized, decellularized-sterilized, fixed, and native porcine pericardial tissue versus native valve leaflet cusps. The results of non-parametric analysis showed statistically significant differences (p < .05) between the stiffness of decellularized versus native pericardium and native cusps as well as fixed tissue, respectively; however, decellularized tissue showed large increases in elastic properties. Porosity testing of the tissues showed no statistical difference between decellularized and decell-sterilized tissue compared with native cusps (p > .05). Scanning electron microscopy confirmed that valvular endothelial and interstitial cells colonized the decellularized pericardial surface when seeded and grown for 30 days in static culture. Collagen assays and...Continue Reading

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Citations

Mar 16, 2019·Journal of Biomechanical Engineering·Christopher NobleMelissa Young
Aug 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tanveer Ahmed ShaikJürgen Popp
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Methods Mentioned

BETA
xenograft
scanning
transmission electron microscopy
light microscopy
ELISA
xenografts
transgenic

Software Mentioned

SAS
Matlab
JMP Pro
JMP

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