Biomechanical and ultrastructural comparison of cryopreservation and a novel cellular extraction of porcine aortic valve leaflets

Journal of Biomedical Materials Research
D W CourtmanG Wilson

Abstract

Heart valve substitutes of biological origin often fail by degenerative mechanisms. Many authors have hypothesized that mechanical fatigue and structural degradation are instrumental to in vivo failure. Since the properties of the structural matrix at implantation may predetermine failure, we have examined the ultrastructure, fracture, mechanics, and uniaxial high-strain-rate viscoelastic properties of: (1) fresh, (2) cryopreserved, and (3) cellular extracted porcine aortic valve leaflets. The cellular extraction process is being developed in order to reduce immunological attack and calcification. Cryopreservation causes cellular disruption and necrotic changes throughout the tissue, whereas extraction removes all cells and lipid membranes. Both processes leave an intact collagen and elastin structural matrix and preserve the high-strain-rate viscoelastic characteristics of the fresh leaflets. Extraction does cause a 20% reduction in the fracture tension and increases tissue extensibility, with the percent strain at fracture rising to 45.3 +/- 4 (mean +/- SEM) from 31.5 +/- 3 for fresh leaflets. However, extraction does preserve matrix structure and mechanics over the physiological loading range. Glutaraldehyde fixation produce...Continue Reading

References

Jan 1, 1978·Biomaterials, Medical Devices, and Artificial Organs·M ChongY F Missirlis
Oct 1, 1991·Biomaterials·R J LevyJ B Gavin
Apr 1, 1986·The Thoracic and Cardiovascular Surgeon·E BodnarJ Dobrin
Jun 1, 1987·Journal of Biomedical Materials Research·M E NimniK Sheikh
Feb 1, 1988·Journal of Biomedical Materials Research·C E Crofts, E A Trowbridge
Dec 1, 1987·The Annals of Thoracic Surgery·J W KirklinR W Stewart
Mar 1, 1983·The Annals of Thoracic Surgery·G I FiddlerG J Williams
Jun 1, 1994·Journal of Biomedical Materials Research·D W CourtmanG J Wilson

❮ Previous
Next ❯

Citations

Jul 9, 1999·Journal of Biomedical Materials Research·V SamouillanM Spina
Mar 6, 2008·Cell and Tissue Banking·M Esther Rendal VázquezC Andión Núñez
Jun 16, 2004·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Hiroo ShikataJunichi Matsubara
Jan 17, 2004·The Annals of Thoracic Surgery·M Esther Rendal VázquezC Andión Nuñez
Jun 13, 2003·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·Harun ArbatliHugo Vanermen
Jul 1, 1997·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·N R VyavahareR J Levy
Sep 12, 2000·Journal of Biomaterials Science. Polymer Edition·V SamouillanM Spina
Mar 8, 2013·Journal of Biomedical Materials Research. Part a·Selda GoktasPeter S McFetridge
Jul 11, 2008·Journal of Biomedical Materials Research. Part a·Thomas CrouzierPeter S McFetridge
Jul 1, 2004·Journal of Biomedical Materials Research. Part a·Peter S McFetridgeJulian B Chaudhuri
Oct 15, 2005·Circulation Research·Ivan Vesely
Jan 28, 2005·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Peter S McFetridgeJulian B Chaudhuri
Jul 4, 2006·Journal of Biomedical Materials Research. Part a·Steven R MeyerDavid B Ross
Jun 23, 2005·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Joel DanielPeter S McFetridge
Feb 7, 2007·Expert Review of Medical Devices·Michael S SacksPaul Schmidt
Nov 24, 1999·Journal of Biomedical Materials Research·J S Cartmell, M G Dunn
Sep 14, 2006·The International Journal of Artificial Organs·Y UedaH Matsuda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi
I KashimaS Kawada
© 2021 Meta ULC. All rights reserved