During developmental stages, biomechanical stimuli on cardiac cells modulate genetic programs, and deviations from normal stimuli can lead to cardiac defects. Therefore, it is important to characterize normal cardiac biomechanical stimuli during early developmental stages. Using the chicken embryo model of cardiac development, we focused on characterizing biomechanical stimuli on the Hamburger-Hamilton (HH) 18 chick cardiac outflow tract (OFT), the distal portion of the heart from which a large portion of defects observed in humans originate. To characterize biomechanical stimuli in the OFT, we used a combination of in vivo optical coherence tomography (OCT) imaging, physiological measurements and computational fluid dynamics (CFD) modeling. We found that, at HH18, the proximal portion of the OFT wall undergoes larger circumferential strains than its distal portion, while the distal portion of the OFT wall undergoes larger wall stresses. Maximal wall shear stresses were generally found on the surface of endocardial cushions, which are protrusions of extracellular matrix onto the OFT lumen that later during development give rise to cardiac septa and valves. The non-uniform spatial and temporal distributions of stresses and strai...Continue Reading
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Synergistic effects of fluid shear stress and cyclic circumferential stretch on vascular endothelial cell morphology and cytoskeleton
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Remodeling of chick embryonic ventricular myoarchitecture under experimentally changed loading conditions
Interaction between wall shear stress and circumferential strain affects endothelial cell biochemical production
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Secondary heart field contributes myocardium and smooth muscle to the arterial pole of the developing heart
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Transitions in early embryonic atrioventricular valvular function correspond with changes in cushion biomechanics that are predictable by tissue composition
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Changes in wall motion and blood flow in the outflow tract of chick embryonic hearts observed with optical coherence tomography after outflow tract banding and vitelline-vein ligation
Measurements of the wall shear stress distribution in the outflow tract of an embryonic chicken heart
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Measurement of absolute blood flow velocity in outflow tract of HH18 chicken embryo based on 4D reconstruction using spectral domain optical coherence tomography
Blood flow dynamics of one cardiac cycle and relationship to mechanotransduction and trabeculation during heart looping
Blood flow dynamics reflect degree of outflow tract banding in Hamburger-Hamilton stage 18 chicken embryos
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Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography
Using optical coherence tomography to rapidly phenotype and quantify congenital heart defects associated with prenatal alcohol exposure
Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos
Dynamic imaging and quantitative analysis of cranial neural tube closure in the mouse embryo using optical coherence tomography
A method to quantify mechanobiologic forces during zebrafish cardiac development using 4-D light sheet imaging and computational modeling
Changes in dynamic embryonic heart wall motion in response to outflow tract banding measured using video densitometry
A method to study the hemodynamics of chicken embryo's aortic arches using optical coherence tomography
Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease
4-D Computational Modeling of Cardiac Outflow Tract Hemodynamics over Looping Developmental Stages in Chicken Embryos.
Organ Dynamics and Hemodynamic of the Whole HH25 Avian Embryonic Heart, Revealed by Ultrasound Biomicroscopy, Boundary Tracking, and Flow Simulations
Embryonic aortic arch hemodynamics are a functional biomarker for ethanol-induced congenital heart defects [Invited
Increased Hemodynamic Load in Early Embryonic Stages Alters Myofibril and Mitochondrial Organization in the Myocardium
Alterations in pulse wave propagation reflect the degree of outflow tract banding in HH18 chicken embryos
Organ Dynamics and Fluid Dynamics of the HH25 Chick Embryonic Cardiac Ventricle as Revealed by a Novel 4D High-Frequency Ultrasound Imaging Technique and Computational Flow Simulations
Computational Modeling of Blood Flow Hemodynamics for Biomechanical Investigation of Cardiac Development and Disease.
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