Biomimetic Concealing of PLGA Microspheres in a 3D Scaffold to Prevent Macrophage Uptake

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Silvia MinardiEnnio Tasciotti

Abstract

Scaffolds functionalized with delivery systems for the release of growth factors is a robust strategy to enhance tissue regeneration. However, after implantation, macrophages infiltrate the scaffold, eventually initiating the degradation and clearance of the delivery systems. Herein, it is hypothesized that fully embedding the poly(d,l-lactide-co-glycolide acid) microspheres (MS) in a highly structured collagen-based scaffold (concealing) can prevent their detection, preserving the integrity of the payload. Confocal laser microscopy reveals that non-embedded MS are easily internalized; when concealed, J774 and bone marrow-derived macrophages (BMDM) cannot detect them. This is further demonstrated by flow cytometry, as a tenfold decrease is found in the number of MS engulfed by the cells, suggesting that collagen can cloak the MS. This correlates with the amount of nitric oxide and tumor necrosis factor-α produced by J774 and BMDM in response to the concealed MS, comparable to that found for non-functionalized collagen scaffolds. Finally, the release kinetics of a reporter protein is preserved in the presence of macrophages, only when MS are concealed. The data provide detailed strategies for fabricating three dimensional (3D) b...Continue Reading

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Citations

Mar 10, 2016·Annals of Biomedical Engineering·Silvia MinardiEnnio Tasciotti
Oct 7, 2017·Frontiers in Bioengineering and Biotechnology·Bruna CorradettiEnnio Tasciotti
Dec 7, 2017·Scientific Reports·Bruna CorradettiEnnio Tasciotti
Jul 12, 2018·Advanced Healthcare Materials·Francesca TaraballiEnnio Tasciotti
Feb 10, 2019·The Journal of Pharmacology and Experimental Therapeutics·Bruna CorradettiMauro Ferrari
Oct 22, 2020·International Journal of Molecular Sciences·Antonia MancusoDonatella Paolino
Apr 9, 2021·Acta Biomaterialia·Shue JinJidong Li
Mar 24, 2018·ACS Applied Materials & Interfaces·Pasquale SaccoGianluca Turco

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