Biomimetic engineering of conductive curli protein films

Nanotechnology
Noémie-Manuelle Dorval CourchesneNeel S Joshi

Abstract

Bioelectronic systems derived from peptides and proteins are of particular interest for fabricating novel flexible, biocompatible and bioactive devices. These synthetic or recombinant systems designed for mediating electron transport often mimic the proteinaceous appendages of naturally occurring electroactive bacteria. Drawing inspiration from such conductive proteins with a high content of aromatic residues, we have engineered a fibrous protein scaffold, curli fibers produced by Escherichia coli bacteria, to enable long-range electron transport. We report the genetic engineering and characterization of curli fibers containing aromatic residues of different nature, with defined spatial positioning, and with varying content on single self-assembling CsgA curli subunits. Our results demonstrate the impressive versatility of the CsgA protein for genetically engineering protein-based materials with new functions. Through a scalable purification process, we show that macroscopic gels and films can be produced, with engineered thin films exhibiting a greater conductivity compared with wild-type curli films. We anticipate that this engineered conductive scaffold, and our approach that combines computational modeling, protein engineer...Continue Reading

References

Aug 29, 2006·Applied and Environmental Microbiology·Gemma RegueraDerek R Lovley
Apr 1, 2008·Cellular Microbiology·Elisabeth Ashman Epstein, Matthew R Chapman
Jul 4, 2009·The Journal of Biological Chemistry·Frank ShewmakerReed B Wickner
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xuan WangMatthew R Chapman
Apr 5, 2011·Annual Review of Biochemistry·Brandon H Toyama, Jonathan S Weissman
Aug 9, 2011·Nature Nanotechnology·Nikhil S MalvankarDerek R Lovley
Sep 7, 2011·Structure·Jonathan D TaylorSteve J Matthews
Nov 28, 2012·Advanced Healthcare Materials·Meredith Muskovich, Christopher J Bettinger
Aug 9, 2011·Advanced Functional Materials·Heather A CurrieDavid L Kaplan
Aug 24, 2013·The Journal of Biological Chemistry·Patrick N Reardon, Karl T Mueller
Mar 22, 2014·Advanced Materials·Noémie-Manuelle Dorval CourchesnePaula T Hammond
Mar 25, 2014·Nature Materials·Allen Y ChenTimothy K Lu
Apr 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Yinan LinDavid L Kaplan
May 16, 2014·Chemical Communications : Chem Comm·Denis IvnitskiGonen Ashkenasy
May 28, 2014·Current Opinion in Biotechnology·Nikhil S Malvankar, Derek R Lovley
Sep 18, 2014·Nature Communications·Peter Q NguyenNeel S Joshi
Nov 22, 2014·Journal of the American Chemical Society·Pengfei TianKresten Lindorff-Larsen
Mar 5, 2015·MBio·Nikhil S MalvankarDerek R Lovley
Jan 7, 2016·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Alyssa Y ZhouMichel M Maharbiz
Mar 25, 2016·Scientific Reports·Sanela Lampa-PastirkGemma Reguera
Jul 14, 2016·Small·Yang TanDerek R Lovley
Aug 31, 2016·Nature Reviews. Microbiology·Liang ShiJames K Fredrickson
Mar 11, 2017·Nature Chemistry·Lucie AltamuraVincent Forge
Apr 9, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·M Tarek AbdelwahabUrartu Ozgur Safak Seker
May 13, 2017·Biochemistry·Hanliu WangMichael L Gross
Mar 15, 2018·ACS Nano·Nicole L IngAllon I Hochbaum
May 8, 2017·ACS Biomaterials Science & Engineering·Noémie-Manuelle Dorval CourchesneNeel S Joshi

❮ Previous
Next ❯

Citations

Apr 21, 2019·Applied and Environmental Microbiology·Anton KanNeel S Joshi
Apr 18, 2019·MBio·David J F WalkerDerek R Lovley
Aug 14, 2019·Advanced Materials·Ebuzer KalyoncuUrartu Ozgur Safak Seker
Dec 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Catharine ShippsNikhil S Malvankar
Nov 26, 2020·Trends in Biotechnology·Wil V Srubar
Mar 5, 2021·Biofabrication·Guy DecanteJ Miguel Oliveira
May 18, 2021·Advanced Functional Materials·Avinash Manjula-BasavannaNeel S Joshi
Jul 2, 2021·Materials Today. Bio·O Burgos-MoralesW Weber
Nov 26, 2020·ACS Synthetic Biology·Zahra AbdaliNoémie-Manuelle Dorval Courchesne
Dec 15, 2020·ACS Biomaterials Science & Engineering·Austin J GrahamBenjamin K Keitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.