Biomimetic scaffolds and dynamic compression enhance the properties of chondrocyte- and MSC-based tissue-engineered cartilage

Journal of Tissue Engineering and Regenerative Medicine
Nopporn SawatjuiTravis Klein

Abstract

Adult chondrocytes are surrounded by a protein- and glycosaminoglycan-rich extracellular matrix and are subjected to dynamic mechanical compression during daily activities. The extracellular matrix and mechanical stimuli play an important role in chondrocyte biosynthesis and homeostasis. In this study, we aimed to develop scaffold and compressive loading conditions that mimic the native cartilage micro-environment and enable enhanced chondrogenesis for tissue engineering applications. Towards this aim, we fabricated porous scaffolds based on silk fibroin (SF) and SF with gelatin/chondroitin sulfate/hyaluronate (SF-GCH), seeded the scaffolds with either human bone marrow mesenchymal stromal cells (BM-MSCs) or chondrocytes, and evaluated their performance with and without dynamic compression. Human chondrocytes derived from osteoarthritic joints and BM-MSCs were seeded in scaffolds, precultured for 1 week, and subjected to compression with 10% dynamic strain at 1 Hz, 1 hr/day for 2 weeks. When dynamic compression was applied, chondrocytes significantly increased expression of aggrecan (ACAN) and collagen X (COL10A1) up to fivefold higher than free-swelling controls. In addition, dynamic compression dramatically improved the chond...Continue Reading

References

Jun 13, 1998·Archives of Biochemistry and Biophysics·W B ValhmuA Ratcliffe
Oct 18, 2001·Clinical Orthopaedics and Related Research·R D CouttsD Amiel
Oct 19, 2001·Clinical Orthopaedics and Related Research·K W LiR L Sah
Aug 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Twana DavissonAnthony Ratcliffe
Mar 30, 2004·Journal of Biomechanics·John D KisidayAlan J Grodzinsky
Jan 27, 2005·Stem Cells·Deborah PhilpHynda K Kleinman
Jan 30, 2007·Biomaterials·Rhima M ColemanRobert E Guldberg
May 31, 2008·Journal of Tissue Engineering and Regenerative Medicine·Chelsea N Salinas, Kristi S Anseth
Oct 22, 2008·Biomaterials·Natalja E FedorovichWouter J A Dhert
May 13, 2009·Journal of Cellular and Molecular Medicine·Zhen LiMartin J Stoddart
Jan 29, 2011·Biotechnology and Bioengineering·Scott C TranSteven H Elder
Nov 22, 2011·Tissue Engineering. Part a·Karsten SchrobbackTravis Jacob Klein
May 9, 2012·Current Stem Cell Research & Therapy·Nigel MabvuureWasim S Khan
Sep 4, 2013·Journal of Biomedical Materials Research. Part a·Peter A LevettTravis J Klein
May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Nopporn SawatjuiTemduang Limpaiboon

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Citations

Sep 15, 2020·Journal of Tissue Engineering·Hanxiang LeJianxun Ding
Oct 9, 2020·Stem Cells International·Renwang ShengJialin Chen
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Sarah DavisTosca Roncada
Jan 8, 2020·ACS Applied Materials & Interfaces·Felipe Olate-MoyaHumberto Palza
Aug 13, 2018·ACS Biomaterials Science & Engineering·Gu ChengQun Wang

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