Biomimetic tendon extracellular matrix composite gradient scaffold enhances ligament-to-bone junction reconstruction

Acta Biomaterialia
Huanhuan LiuHongwei Ouyang

Abstract

Management of ligament/tendon-to-bone-junction healing remains a formidable challenge in the field of orthopedic medicine to date, due to deficient vascularity and multi-tissue transitional structure of the junction. Numerous strategies have been employed to improve ligament-bone junction healing, including delivery of stem cells, bioactive factors, and synthetic materials, but these methods are often inadequate at recapitulating the complex structure-function relationships at native tissue interfaces. Here, we developed an easily-fabricated and effective biomimetic composite to promote the regeneration of ligament-bone junction by physically modifying the tendon extracellular matrix (ECM) into a Random-Aligned-Random composite using ultrasound treatment. The differentiation potential of rabbit bone marrow stromal cells on the modified ECM were examined in vitro. The results demonstrated that the modified ECM enhanced expression of chondrogenesis and osteogenesis-associated epigenetic genes (Jmjd1c, Kdm6b), transcription factor genes (Sox9, Runx2) and extracellular matrix genes (Col2a1, Ocn), resulting in higher osteoinductivity than the untreated tendon ECM in vitro. In the rabbit anterior cruciate ligament (ACL) reconstructio...Continue Reading

References

Mar 8, 2006·Biomaterials·Thomas W GilbertStephen F Badylak

❮ Previous
Next ❯

Citations

Nov 14, 2018·Tissue Engineering. Part C, Methods·Luis Diaz-GomezAntonios G Mikos
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Fengxuan HanBin Li
Apr 30, 2019·Small·Marcel Alexander HeinrichYu Shrike Zhang
Jan 26, 2021·ACS Biomaterials Science & Engineering·Behzad Shiroud HeidariBarry Doyle
Mar 6, 2021·Bioactive Materials·Tingyun LeiZi Yin
Mar 9, 2021·Frontiers in Bioengineering and Biotechnology·Elisa MazzoniFernanda Martini
Apr 2, 2021·Frontiers in Bioengineering and Biotechnology·Zimu MaoJiakuo Yu
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Zhuyan JiangMeifeng Zhu
Aug 11, 2021·Tissue Engineering. Part a·Lewis S GaffneyDonald O Freytes
Nov 17, 2021·ACS Biomaterials Science & Engineering·Carlos Pitta KruizeMohammad J Mirzaali

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.