Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy

Photochemistry and Photobiology
Fernanda G BassoJosimeri Hebling

Abstract

This study evaluated the effects of LLLT on the expression of inflammatory cytokines related to the development of oral mucositis by gingival fibroblasts. Primary gingival fibroblasts were seeded on 24-well plates (10(5) cells/well) for 24 h. Fresh serum-free culture medium (DMEM) was then added, and cells were placed in contact with LPS (Escherichia coli, 1 μg mL(-1)), followed by LLLT irradiation (LaserTABLE-InGaAsP diode prototype-780 nm, 25 mW) delivering 0, 0.5, 1.5 or 3 J cm(-2)². Cells without contact with LPS were also irradiated with the same energy densities. Gene expression of TNF-α, IL-1β, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines was determined by enzyme-linked immunosorbent (ELISA) assay. Data were statistically analyzed by the Kruskal-Wallis test, complemented by the Mann-Whitney test (P < 0.05). LPS treatment increased the gene expression and protein synthesis of TNF-α, IL-6 and IL-8, while the expression of IL-1β was not affected. For LPS-treated groups, LLLT promoted significant decreases in the expression of TNF-α, IL-6, and IL-8 at 1.5 J cm(-2) and 3 J cm(-2). These results demonstrate that LLLT promoted a beneficial biomodulatory effect on the expression of infla...Continue Reading

References

Dec 11, 1992·Oral Surgery, Oral Medicine, and Oral Pathology·S T SonisM Nicolson
Apr 27, 2004·Cancer·Stephen T SonisUNKNOWN International Society for Oral Oncology
Nov 11, 2005·The British Journal of Dermatology·T OmiK Thestrup-Pedersen
Mar 1, 2006·Photomedicine and Laser Surgery·F AimbireJ M Bjordal
Jan 20, 2007·Cancer·Dorothy M KeefeUNKNOWN Mucositis Study Section of the Multinational Association of Supportive Care in Cancer and the International Society for Oral
Feb 6, 2007·Clinics in Dermatology·Jie LiRobert Kirsner
Feb 6, 2007·Clinics in Dermatology·Nathan B MenkeRobert F Diegelmann
Mar 30, 2007·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Mark M SchubertMohd Hamdi
Apr 7, 2007·Current Opinion in Otolaryngology & Head and Neck Surgery·Nathaniel Treister, Stephen Sonis
Apr 26, 2007·Lasers in Surgery and Medicine·Fernanda P EduardoMárcia M Marques
Jan 17, 2008·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Arsinoi XanthinakiEfstratios Patsouris
May 27, 2008·Journal of Dental Research·P PalmqvistU H Lerner
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stephan BarrientosMarjana Tomic-Canic
Mar 11, 2009·Journal of Biomedical Science·Xuejuan Gao, Da Xing
Oct 3, 2009·Photomedicine and Laser Surgery·Marica Simunović-SoskićIvana Miletić
Feb 9, 2010·Journal of Dental Research·S Guo, L A Dipietro
May 28, 2010·Journal of Dental Research·P P C SouzaU H Lerner
Oct 26, 2010·Photomedicine and Laser Surgery·Geisa Badauy Lauria SilvaMaria Alves Garcia Silva
May 4, 2011·Brazilian Dental Journal·Maíra Dória Martinez da Costa LinoLuciana Maria Pedreira Ramalho
Jun 11, 2011·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Jan Magnus BjordalRodrigo Ab Lopes-Martins
Sep 17, 2011·International Journal of Paediatric Dentistry·Thais Morales-RojasAlejandra Alvarez
Feb 9, 2012·Lasers in Medical Science·Fernanda G BassoCarlos A de Souza Costa
Jun 26, 2012·Current Pharmaceutical Design·Chiara Gasparini, Marc Feldmann
Jul 17, 2012·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J M Reinke, H Sorg
Jul 31, 2012·International Journal of Dentistry·Fernanda G BassoCarlos A de Souza Costa
Sep 25, 2012·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Cesar MiglioratiUNKNOWN Mucositis Study Group of the Multinational Association of Supportive Care in Cancer/International Society of Oral Oncology (
Oct 10, 2013·Photomedicine and Laser Surgery·Emery C LinsVanderlei S Bagnato
Mar 19, 2014·Journal of Biomedical Optics·Ryan Spitler, Michael W Berns

❮ Previous
Next ❯

Citations

Sep 24, 2015·Journal of Photochemistry and Photobiology. B, Biology·Fernanda Oliveira DuarteNivaldo Antonio Parizotto
Apr 2, 2016·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·A El BousaadaniM Mahtar
Jun 28, 2015·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Supriya MallickG K Rath
Apr 30, 2016·Lasers in Medical Science·Fernanda G BassoJosimeri Hebling
Oct 12, 2016·Lasers in Medical Science·Vanessa Lima Cavalcante de OliveiraPaulo de Tarso Camillo de Carvalho
Mar 13, 2017·Lasers in Medical Science·Fernanda Gonçalves BassoCarlos Alberto de Souza Costa
Dec 22, 2017·Photochemistry and Photobiology·Taisa Nogueira PansaniCarlos Alberto de Souza Costa
Feb 25, 2018·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Jon KirkJean-Luc C Mougeot
Jun 12, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hannah SerrageMichael R Hamblin
Aug 8, 2019·Photobiomodulation, Photomedicine, and Laser Surgery·Osman Tolga HarorliNuray Erin
Aug 4, 2020·Journal of Pharmacy & Bioallied Sciences·Anmar Al-TaieAtaa Altaee
Feb 8, 2020·Lasers in Medical Science·Laís Medeiros CardosoFernanda Gonçalves Basso
Dec 2, 2020·International Journal of Molecular Sciences·Yujin OhsugiTakanori Iwata
May 16, 2021·Lasers in Medical Science·H J SerrageM R Milward
May 23, 2021·Archives of Oral Biology·Laís Medeiros CardosoFernanda Gonçalves Basso
Nov 23, 2020·Archives of Oral Biology·Ammaar H AbidiChristopher J Walinski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved