Biomolecule analysis by ion mobility spectrometry.

Annual Review of Analytical Chemistry
Brian C BohrerDavid E Clemmer

Abstract

Although nonnative protein conformations, including intermediates along the folding pathway and kinetically trapped misfolded species that disfavor the native state, are rarely isolated in the solution phase, they are often stable in the gas phase, where macromolecular ions from electrospray ionization can exist in varying charge states. Differences in the structures of nonnative conformations in the gas phase are often large enough to allow different shapes and charge states to be separated because of differences in their mobilities through a gas. Moreover, gentle collisional activation can be used to induce structural transformations. These new structures often have different mobilities. Thus, there is the possibility of developing a multidimensional separation that takes advantage of structural differences of multiple stable states. This review discusses how nonnative states differ in the gas phase compared with solution and presents an overview of early attempts to utilize and manipulate structures in order to develop ion mobility spectrometry as a rapid and sensitive technique for separating complex mixtures of biomolecules prior to mass spectrometry.

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Citations

Aug 7, 2013·Journal of Structural and Functional Genomics·Eric D MerkleyJoshua N Adkins
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Virginia G GigantiLaurence A Angel
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Zhiyu LiDavid E Clemmer
Dec 2, 2011·Journal of the American Society for Mass Spectrometry·Fabien ChirotPhilippe Dugourd
May 11, 2012·Journal of the American Society for Mass Spectrometry·Florent CalvoPhilippe Dugourd
Jun 6, 2009·Journal of the American Society for Mass Spectrometry·Qin ZhaoEthan R Badman
May 1, 2010·Journal of the American Society for Mass Spectrometry·Qin ZhaoEthan R Badman
May 4, 2010·Journal of the American Society for Mass Spectrometry·Ellen Inutan, Sarah Trimpin
Aug 28, 2009·Analytical Chemistry·Alexandre A ShvartsburgDanielle Toutoungi
Aug 26, 2011·Chemical Reviews·Rajesh ChakrabartyPeter J Stang
Jun 17, 2011·Journal of the American Chemical Society·Linjie HanBrandon T Ruotolo
Jun 8, 2010·Journal of the American Chemical Society·Tony Ly, Ryan R Julian
Dec 6, 2012·The Journal of Physical Chemistry. B·Huilin ShiRenã A S Robinson
Mar 23, 2011·Journal of Proteome Research·Stephen J ValentineDavid E Clemmer
Dec 14, 2011·Journal of Proteome Research·Dragan IsailovicDavid E Clemmer
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Mar 14, 2012·Bioanalysis·J Larry Campbell, J C Yves Le Blanc
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Jan 5, 2014·Journal of the American Society for Mass Spectrometry·Waldemar HoffmannKevin Pagel
Apr 9, 2013·Annual Review of Analytical Chemistry·Corey N StedwellNicolas C Polfer
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Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Christopher J Hogan, Juan Fernández de la Mora
Mar 25, 2014·Journal of the American Society for Mass Spectrometry·Huilin ShiDavid E Clemmer
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Feb 27, 2013·Chemical Reviews·Yaoyang ZhangJohn R Yates
Apr 25, 2013·Physical Chemistry Chemical Physics : PCCP·Molly T SoperBrandon T Ruotolo
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guanbo WangIgor A Kaltashov
Mar 30, 2013·International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry·Linjie Han, Brandon T Ruotolo
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Brian C Bohrer, David E Clemmer
Oct 22, 2011·Journal of the American Society for Mass Spectrometry·Anastasia KharlamovaScott A McLuckey
Feb 5, 2016·Analytical Chemistry·Nicole Andrea MeyerGuillermo Vidal-de-Miguel
Apr 2, 2014·Journal of Proteomics·Megan M GesselRichard M Caprioli
Feb 11, 2016·Journal of the American Society for Mass Spectrometry·Matthew S GloverDavid E Clemmer
May 16, 2015·Mass Spectrometry Reviews·Alexandra Tsybizova, Jana Roithová
Mar 26, 2013·International Journal of Mass Spectrometry·Benjamin N MooreRyan R Julian
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Jun 26, 2015·Analytical Chemistry·Martin F CzarRebecca A Jockusch
Sep 13, 2015·Journal of the American Society for Mass Spectrometry·Liuqing ShiDavid E Clemmer

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Methods Mentioned

BETA
nuclear magnetic resonance

Software Mentioned

FAIMS

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