Biomolecules in multilayer film for antimicrobial and easy-cleaning stainless steel surface applications

Biofouling
C VreulsCécile Van de Weerdt

Abstract

Microorganisms are able to attach to, grow on, and ultimately form biofilms on a large variety of surfaces, such as industrial equipment, food contact surfaces, medical implants, prostheses and operating rooms. Once organized into biofilms, bacteria are difficult to remove and kill, which increases the risk of cross-contamination and infection. One way to address the problem may thus be to develop antibacterial, anti-adhesion, 'easy cleaning' surfaces. In this study, stainless steel (SS) surfaces with antibacterial properties were created by embedding several antimicrobial peptides in a multilayer film architecture. The biocidal effect of these surfaces was demonstrated against both Gram-positive and Gram-negative bacteria according to two ISO tests. Also, coating SS surfaces with either mucin or heparin led to a reduction of S. epidermidis adhesion of almost 95% vs the bare substratum. Finally, by combining both antibacterial and anti-adhesion biomolecules in the same multilayer film, SS surfaces with better cleanability were produced. This surface coating property may help to delay the buildup of a dead bacterial layer which is known to progressively reduce exposure of the coating, leading to an undesirable decrease in the an...Continue Reading

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Citations

Aug 28, 2012·Biomacromolecules·Thomas CrouzierKatharina Ribbeck
Nov 2, 2013·Trends in Biotechnology·Mario SalwiczekHelmut Thissen
Feb 12, 2011·Colloids and Surfaces. B, Biointerfaces·Arnaud HéquetClaire-Marie Pradier
Oct 20, 2020·Critical Reviews in Biotechnology·Mehdi Kazemzadeh-NarbatAli Khademhosseini

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Methods Mentioned

BETA
Protein Assay
quartz crystal microbalance
scanning electron microscopy

Software Mentioned

Biocoat

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