Biomonitoring for assessment of organic dust-induced lung inflammation

The European Respiratory Journal
L J Mueller-AnnelingP S Thorne

Abstract

Inhalation exposure to particulate matter containing endotoxin (or lipopolysaccharide (LPS)) occurs in a variety of occupations. Nasal lavage and induced sputum have been used to evaluate lung inflammation resulting from such exposures. Whole blood assay (WBA) measures cytokine production of leukocytes after ex vivo stimulation with LPS. The present study examined the effectiveness of WBA for evaluating inflammatory responses and susceptibility. C3HeB/FEJ mice were tolerised by LPS injection or sham tolerised with saline. Animals then inhaled either swine barn dust extract containing endotoxin or saline. Bronchoalveolar lavage (BAL) fluid was assayed for leukocyte counts and pro-inflammatory cytokines (interleukin-6, tumour necrosis factor-alpha). Whole blood was stimulated with 10 or 100 ng.mL(-1) of LPS, incubated for 5 or 18 h and assayed for cytokines. Barn dust-exposed groups revealed significantly higher total cells, neutrophils and cytokines in BAL compared with saline-exposed groups. Animals tolerised to LPS and exposed to barn dust demonstrated lower cellular and cytokine BAL responses. Similarly, WBA yielded significantly elevated cytokines with barn dust exposure and reduced responses with tolerisation. This study de...Continue Reading

Citations

Oct 30, 2007·Journal of Toxicology and Environmental Health. Part a·S P StraszekP S Thorne
Jun 25, 2010·Journal of Toxicology and Environmental Health. Part a·Jayda CleaveJohn R Gordon
Jan 25, 2008·The European Respiratory Journal·T A WyattD J Romberger
Apr 14, 2007·Environmental Health Perspectives·Vicki H GrassianPeter S Thorne
Oct 30, 2009·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Romy SethSusan M Tarlo
Aug 21, 2014·Journal of Exposure Science & Environmental Epidemiology·D' Ann L WilliamsPatrick N Breysse
Apr 28, 2009·International Journal of Hygiene and Environmental Health·Verena LiebersMonika Raulf-Heimsoth
May 12, 2012·American Journal of Industrial Medicine·Katarina KulhankovaPeter S Thorne
Feb 11, 2010·Journal of Environmental Monitoring : JEM·D TraversiG Gilli
Dec 12, 2012·Innate Immunity·Jakob H BønløkkeCaroline Duchaine
Nov 26, 2010·The Journal of Allergy and Clinical Immunology·Romy SethSusan M Tarlo
Jan 27, 2011·Particle and Fibre Toxicology·Larissa V StebounovaPeter S Thorne

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