Bioorthogonal Click Chemistry-Based Synthetic Cell Glue

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Heebeom KooSeok Hyun Yun

Abstract

Artificial methods of cell adhesion can be effective in building functional cell complexes in vitro, but methods for in vivo use are currently lacking. Here, a chemical cell glue based on bioorthogonal click chemistry with high stability and robustness is introduced. Tetrazine (Tz) and trans-cyclooctene (TCO) conjugated to the cell surface form covalent bonds between cells within 10 min in aqueous conditions. Glued, homogeneous, or heterogeneous cell pairs remain viable and stably attached in a microfluidic flow channel at a shear stress of 20 dyn cm(-2) . Upon intravenous injection of assembled Jurkat T cells into live mice, fluorescence microscopy shows the trafficking of cell pairs in circulation and their infiltration into lung tissues. These results demonstrate the promising potential of chemically glued cell pairs for various applications ranging from delivering therapeutic cells to studying cell-cell interactions in vivo.

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Citations

Oct 28, 2016·Bioconjugate Chemistry·Heebeom KooSeok Hyun Yun
Jan 31, 2018·Angewandte Chemie·Peng ShiYong Wang
Jun 13, 2018·Chemistry : a European Journal·Guhuan LiuShiyong Liu
Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yukiya TakayamaMakiya Nishikawa
Jul 20, 2021·Chemical Science·Chad PlumetSébastien Papot
Jul 2, 2020·ACS Synthetic Biology·Samaneh RasoulinejadSeraphine V Wegner

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