Biophysical analysis of the structural evolution of substrate specificity in RuBisCO

Proceedings of the National Academy of Sciences of the United States of America
Saroj PoudelPaul G Falkowski

Abstract

Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant enzyme on Earth. However, its catalytic rate per molecule of protein is extremely slow and the binding of the primary substrate, CO2, is competitively displaced by O2. Hence, carbon fixation by RuBisCO is highly inefficient; indeed, in higher C3 plants, about 30% of the time the enzyme mistakes CO2 for O2 Using genomic and structural analysis, we identify regions around the catalytic site that play key roles in discriminating between CO2 and O2 Our analysis identified positively charged cavities directly around the active site, which are expanded as the enzyme evolved with higher substrate specificity. The residues that extend these cavities have recently been under selective pressure, indicating that larger charged pockets are a feature of modern RuBisCOs, enabling greater specificity for CO2 This paper identifies a key structural feature that enabled the enzyme to evolve improved CO2 sequestration in an oxygen-rich atmosphere and may guide the engineering of more efficient RuBisCOs.

References

Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Nov 25, 2003·Advances in Protein Chemistry·Jay W Ponder, David A Case
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Guillaume G B TcherkezT John Andrews
Sep 29, 2006·Bioinformatics·Mario A Fares, David McNally
May 15, 2007·BMC Evolutionary Biology·Maxim V Kapralov, Dmitry A Filatov
Jun 15, 2007·Eukaryotic Cell·James V Moroney, Ruby A Ynalvez
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·F Robert TabitaSum Chan
Apr 26, 2008·Journal of Experimental Botany·Martin A J ParryP John Andralojc
May 20, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John A RavenChristina L De La Rocha
Jul 13, 2011·Journal of Molecular Biology·Michiel van LunInger Andersson
Jan 11, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J N YoungD A Filatov
Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Boguslaw Stec
Nov 20, 2012·Journal of Experimental Botany·Martin A J ParrySpencer M Whitney
Feb 6, 2014·Journal of the American Chemical Society·Michiel van LunInger Andersson
Feb 21, 2014·Nature·Timothy W LyonsNoah J Planavsky
Jul 8, 2016·Science Advances·Genming LuoRoger E Summons
Aug 27, 2017·Current Opinion in Biotechnology·Tobias J Erb, Jan Zarzycki
Sep 9, 2017·Protein Science : a Publication of the Protein Society·Fabian Sievers, Desmond G Higgins
Jun 4, 2018·Nucleic Acids Research·Wei TianJie Liang
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yinon M Bar-On, Ron Milo
May 11, 2019·Free Radical Biology & Medicine·Rosalind E M Rickaby, M R Eason Hubbard
Dec 5, 2019·The Journal of Biological Chemistry·António J M RibeiroJanet M Thornton

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Citations

May 18, 2021·Frontiers in Plant Science·Juan José Pierella KarlusichHaimanti Biswas
Jun 18, 2021·Frontiers in Chemistry·Xiaoxiao WangLiming Zhao

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