Biophysical and Biochemical Characterization of Nascent Polypeptide-Associated Complex of Picrophilus torridus and Elucidation of Its Interacting Partners

Frontiers in Microbiology
Neelja SinghalManisha Goel

Abstract

Nascent polypeptide-associated complex (NAC) is a ribosome-associated molecular chaperone which is present only in archaea and eukaryotes. The primary function of NAC is to shield the newly synthesized polypeptide chains from inappropriate interactions with the cytosolic factors. Besides that, NAC has been implicated in diverse biological functions, which suggest that it might be a multifunctional protein. An elaborate study on NAC can provide useful information on protein folding in extreme conditions in which many archaea grow. Thus, in the present study, we have studied the biophysical and the biochemical characteristics of NAC of Picrophilus torridus, an extreme thermoacidophilic archaeon. The study of protein-protein interactions and binding partners of a protein provides useful insights into the new/unreported roles of a protein. Thus, in this study, we have identified the binding partners of NAC in P. torridus. The NAC protein of P. torridus was cloned, expressed, and purified, and its binding partners were isolated by a pull down assay followed by identification with liquid chromatography-mass spectrometry. To the best of our knowledge, this is the first report on the biophysical and the biochemical characterization of ...Continue Reading

References

Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·W Wickner
Nov 1, 1996·The Journal of Biological Chemistry·K RajaramanC M Rao
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·C VetrianiF T Robb
Apr 29, 2000·Cell·B BukauE A Craig
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Jul 20, 2001·Journal of Bioenergetics and Biomembranes·C L Schmidt, L Shaw
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Dec 12, 2002·Cellular and Molecular Life Sciences : CMLS·S RospertM Gautschi
Oct 19, 2005·Cellular and Molecular Life Sciences : CMLS·R D Wegrzyn, E Deuerling
Nov 8, 2005·Biochimica Et Biophysica Acta·Dirk Schneider, Christian L Schmidt
Oct 20, 2006·Nature·Christian M KaiserJosé M Barral
Mar 12, 2008·Biochimica Et Biophysica Acta·Carsten BerndtArne Holmgren
Apr 9, 2008·Archives of Biochemistry and Biophysics·Maria Giulia Bigotti, Anthony R Clarke
Feb 6, 2010·Biochimica Et Biophysica Acta·Anja HoffmannGünter Kramer
Apr 7, 2010·The Journal of Cell Biology·Ansgar KoplinElke Deuerling
Dec 8, 2016·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Apr 2, 2017·FEMS Microbiology Letters·Shikha RaniPeter Lund
Sep 19, 2017·Critical Reviews in Microbiology·Archana SharmaManisha Goel
Feb 2, 2018·Frontiers in Cellular and Infection Microbiology·Rinki KumarGuruprasad R Medigeshi

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
pull down
PCR
electrophoresis
Circular Dichroism
size exclusion chromatography
gel filtration

Software Mentioned

PARAGON
String
BestSel
MASCOT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.