Biophysical and biomolecular determination of cellular age in humans

Nature Biomedical Engineering
Jude M PhillipDenis Wirtz

Abstract

Ageing research has focused either on assessing organ- and tissue-based changes, such as lung capacity and cardiac function, or on changes at the molecular scale such as gene expression, epigenetic modifications and metabolism. Here, by using a cohort of 32 samples of primary dermal fibroblasts collected from individuals between 2 and 96 years of age, we show that the degradation of functional cellular biophysical features-including cell mechanics, traction strength, morphology and migratory potential-and associated descriptors of cellular heterogeneity predict cellular age with higher accuracy than conventional biomolecular markers. We also demonstrate the use of high-throughput single-cell technologies, together with a deterministic model based on cellular features, to compute the cellular age of apparently healthy males and females, and to explore these relationships in cells from individuals with Werner syndrome and Hutchinson-Gilford progeria syndrome, two rare genetic conditions that result in phenotypes that show aspects of premature ageing. Our findings suggest that the quantification of cellular age may be used to stratify individuals on the basis of cellular phenotypes and serve as a biological proxy of healthspan.

References

Jul 1, 1976·Social Science & Medicine·I Waldron
Jan 1, 1983·Experimental Aging Research·V P Voitenko, A V Tokar
Sep 1, 1980·Mechanisms of Ageing and Development·L Hayflick
Oct 24, 2001·Experimental Gerontology·J CampisiM Rubio
Jan 8, 2004·Annals of Medicine·Donald E Ingber
Jul 5, 2005·Biochemical and Biophysical Research Communications·Yiider TsengDenis Wirtz
Jan 6, 2006·Cleveland Clinic Journal of Medicine·Sara Espinoza, Jeremy D Walston
Jan 31, 2006·Proceedings of the National Academy of Sciences of the United States of America·Noriyuki MiyoshiEarl R Stadtman
Aug 2, 2007·Nature Reviews. Molecular Cell Biology·Judith Campisi, Fabrizio d'Adda di Fagagna
Nov 17, 2007·Aging Cell·Olga A SedelnikovaWilliam M Bonner
Jan 30, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Milan Makale
Oct 9, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Eitaro Nakamura, Kenji Miyao
May 1, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Benjamin D SmithThomas A Buchholz
May 7, 2009·Annual Review of Biophysics·Denis Wirtz
Oct 17, 2009·Current Opinion in Chemical Biology·Mario NiepelPeter K Sorger
Oct 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shyam B KhatauDenis Wirtz
Nov 10, 2009·Ageing Research Reviews·Maria N Starodubtseva
Jan 19, 2010·Annual Review of Pathology·Jean-Philippe CoppéJudith Campisi
May 18, 2010·Nature Cell Biology·Stephanie I FraleyDenis Wirtz
May 19, 2010·Cell·Steven J Altschuler, Lani F Wu
Dec 24, 2010·Aging Cell·Jill A KreilingJohn M Sedivy
Dec 24, 2010·Journal of Visualized Experiments : JoVE·Yvonne Aratyn-SchausMargaret L Gardel
Feb 16, 2011·The Journal of Cell Biology·Francis Rodier, Judith Campisi
Jun 28, 2011·Nature Reviews. Cancer·Denis WirtzPeter C Searson
Jul 7, 2011·PloS One·Sven BocklandtEric Vilain
Nov 22, 2011·Clinics in Plastic Surgery·Christian SchulzeJosef A Käs
Jan 10, 2012·Nature Protocols·Pei-Hsun WuDenis Wirtz
Nov 13, 2012·Annual Review of Physiology·Judith Campisi
Jan 16, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Wei-Chiang ChenDenis Wirtz
Mar 5, 2013·The Journal of Clinical Investigation·Ana Bratic, Nils-Göran Larsson
Mar 30, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Allison B ChamblissDenis Wirtz
Jun 12, 2013·Cell·Carlos López-OtínGuido Kroemer
Sep 3, 2013·Journal of Cellular Physiology·Koh Meng Aw YongRobert H Getzenberg
Oct 22, 2013·Genome Biology·Steve Horvath
Dec 21, 2013·Current Biology : CB·Philipp Isermann, Jan Lammerding
Mar 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pei-Hsun WuDenis Wirtz

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Citations

Jun 11, 2020·Soft Matter·Blanca González-BermúdezGustavo R Plaza
Aug 9, 2020·Experimental Dermatology·Abigail De La PenaChelsey S Simmons
Dec 21, 2018·Genome Biology·Jason G FleischerSaket Navlakha
Apr 6, 2019·Biological Chemistry·Chrysa NikopoulouPeter Tessarz
Jul 25, 2019·Scientific Reports·Peter LenartJulie Bienertová-Vašků
Apr 29, 2018·Scientific Reports·Krungchanuchat SaowalakPilapong Chalermchai
Feb 6, 2020·Science Advances·Pei-Hsun WuDenis Wirtz
Jul 22, 2018·Aging Cell·Emiliano ZamponiPablo Helguera
Nov 25, 2020·Annals of the New York Academy of Sciences·Apratim BajpaiWeiqiang Chen
Jan 21, 2021·Communications Biology·Jude M PhillipDenis Wirtz
Nov 7, 2020·Scientific Reports·Yaelim Lee, G V Shivashankar
Apr 26, 2021·Trends in Biotechnology·Kelvin C M LeeKevin K Tsia
Aug 21, 2021·Aging Cell·Daniel J Simpson, Tamir Chandra
Jan 19, 2021·ACS Biomaterials Science & Engineering·Peiran ZhuYubing Sun
Jul 30, 2019·ACS Chemical Neuroscience·Isara PhiwchaiChalermchai Pilapong
Sep 22, 2021·Nature Communications·Shobana V StassenKevin K Tsia
Nov 2, 2021·Disease Models & Mechanisms·Ineke DhondtWinnok H De Vos

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