Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases.

Retrovirology
Maximilian J HartlBirgitta M Wöhrl

Abstract

The foamy virus Pol protein is translated independently from Gag using a separate mRNA. Thus, in contrast to orthoretroviruses no Gag-Pol precursor protein is synthesized. Only the integrase domain is cleaved off from Pol resulting in a mature reverse transcriptase harboring the protease domain at the N-terminus (PR-RT). Although the homology between the PR-RTs from simian foamy virus from macaques (SFVmac) and the prototype foamy virus (PFV), probably originating from chimpanzee, exceeds 90%, several differences in the biophysical and biochemical properties of the two enzymes have been reported (i.e. SFVmac develops resistance to the nucleoside inhibitor azidothymidine (AZT) whereas PFV remains AZT sensitive even if the resistance mutations from SFVmac PR-RT are introduced into the PFV PR-RT gene). Moreover, contradictory data on the monomer/dimer status of the foamy virus protease have been published. We set out to purify and directly compare the monomer/dimer status and the enzymatic behavior of the two wild type PR-RT enzymes from SFVmac and PFV in order to get a better understanding of the protein and enzyme functions. We determined kinetic parameters for the two enzymes, and we show that PFV PR-RT is also a monomeric prot...Continue Reading

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Citations

May 7, 2010·Medical Microbiology and Immunology·Axel Rethwilm
Feb 18, 2011·Journal of Virology·Maximilian J HartlBirgitta M Wöhrl
Feb 14, 2012·Retrovirology·Berit LeoBirgitta M Wöhrl
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm
Mar 23, 2013·Viruses·Eun-Gyung LeeMaxine L Linial
Oct 15, 2013·Current Opinion in Virology·Axel Rethwilm
May 7, 2014·Antimicrobial Agents and Chemotherapy·Angela CoronaEnzo Tramontano
Jan 3, 2012·Journal of Biomolecular Structure & Dynamics·Maximilian J HartlStephan Schwarzinger
Jul 8, 2021·Journal of Virology·Marzena NowackaMarcin Nowotny

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Methods Mentioned

BETA
NMR
gel filtration
circular dichroism
Size exclusion chromatography
electrophoresis
circular

Software Mentioned

Jasco Spectra Manager
CDSSTR

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