Biophysical and structural characterization of a sequence-diverse set of solute-binding proteins for aromatic compounds.

The Journal of Biological Chemistry
Ruth PietriLisa M Miller

Abstract

Rhodopseudomonas palustris metabolizes aromatic compounds derived from lignin degradation products and has the potential for bioremediation of xenobiotic compounds. We recently identified four possible solute-binding proteins in R. palustris that demonstrated binding to aromatic lignin monomers. Characterization of these proteins in the absence and presence of the aromatic ligands will provide unprecedented insights into the specificity and mode of aromatic ligand binding in solute-binding proteins. Here, we report the thermodynamic and structural properties of the proteins with aromatic ligands using isothermal titration calorimetry, small/wide angle x-ray scattering, and theoretical predictions. The proteins exhibit high affinity for the aromatic substrates with dissociation constants in the low micromolar to nanomolar range. The global shapes of the proteins are characterized by flexible ellipsoid-like structures with maximum dimensions in the 80-90-Å range. The data demonstrate that the global shapes remained unaltered in the presence of the aromatic ligands. However, local structural changes were detected in the presence of some ligands, as judged by the observed features in the wide angle x-ray scattering regime at q ~0.2...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 7, 1994·Biochemistry·M GersteinC Chothia
Jan 1, 1996·Annual Review of Microbiology·C S Harwood, R E Parales
Jun 25, 2004·Nucleic Acids Research·David E KimDavid Baker
Nov 23, 2007·Journal of Molecular Biology·Lee MakowskiRobert F Fischetti
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Oct 22, 2008·Nucleic Acids Research·Florian KieferTorsten Schwede
Oct 30, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kaspar P Locher
Feb 24, 2009·Nature Reviews. Molecular Cell Biology·Douglas C ReesOded Lewinson
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
Jan 6, 2010·Journal of Structural and Functional Genomics·Lee Makowski
Feb 2, 2010·Protein Science : a Publication of the Protein Society·David A Jacques, Jill Trewhella
Mar 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sum ChanL Jeanne Perry
Jun 19, 2010·Journal of Structural Biology·Haydyn D T Mertens, Dmitri I Svergun
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Dec 21, 2010·Journal of Synchrotron Radiation·Marc Allaire, Lin Yang
Aug 10, 2011·BMC Genomics·Sarah E GiulianiFrank R Collart

❮ Previous
Next ❯

Citations

May 21, 2015·Biophysical Journal·Po-Chia Chen, Jochen S Hub
Nov 6, 2012·Current Opinion in Biotechnology·Eduardo DíazJuan Nogales
Dec 18, 2019·Journal of Bacteriology·Jannes VagtsRalf Rabus
Feb 1, 2017·Biochemistry and Biophysics Reports·Elddie Román-MoralesJuan López-Garriga
Mar 21, 2021·Applied and Environmental Microbiology·Jannes VagtsRalf Rabus
Oct 8, 2021·Critical Reviews in Biotechnology·Diogo A M AlexandrinoMaria F Carvalho

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.