Biophysical and structural insight into the USP8/14-3-3 interaction

FEBS Letters
Federica CentorrinoChristian Ottmann

Abstract

The ubiquitin-specific protease 8 (USP8)/14-3-3 protein-protein interaction has recently been shown to exert a significant role in the pathogenesis of Cushing's disease (CD). USP8 is a deubiquitinase that prevents epidermal growth factor receptor (EGFR) degradation. Impairment of 14-3-3 binding leads to a higher deubiquitination of EGFR and results in a higher EGFR signaling and an increased production of adrenocorticotropic hormone. Here we report the high-resolution crystal structure of the 14-3-3 binding motif of USP8 surrounding Ser718 in complex with 14-3-3ζ and characterize the interaction with fluorescence polarization and isothermal titration calorimetry. Furthermore, we analyze the effect of USP8 mutations identified in CD on binding to 14-3-3.

References

Oct 7, 1994·Cell·A Ciechanover
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Apr 29, 2005·Cell Research·Paulette Mhawech
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiaowen YangJonathan M Elkins
Jun 6, 2009·Annual Review of Biochemistry·Francisca E Reyes-TurcuKeith D Wilkinson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Sep 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Benjamin SchumacherChristian Ottmann
Mar 15, 2011·The Biochemical Journal·Lorenzo CamoniPatrizia Aducci
Mar 19, 2011·Cell·Marc VidalAlbert-László Barabási
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Sep 17, 2011·Journal of Chemical Information and Modeling·Roman A Laskowski, Mark B Swindells
Sep 17, 2011·Seminars in Cell & Developmental Biology·Tomas Obsil, Veronika Obsilova
Nov 23, 2011·The Journal of Clinical Investigation·Hidenori FukuokaShlomo Melmed
Dec 14, 2011·The FEBS Journal·Manuela MolzanChristian Ottmann
Dec 26, 2012·Bioorganic & Medicinal Chemistry·Christian Ottmann
Jan 22, 2013·Cellular Signalling·Inez M J MeijerJeroen E M van Leeuwen
Feb 21, 2013·Nature Chemistry·David BierChristian Ottmann
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov
Feb 7, 2014·Database : the Journal of Biological Databases and Curation·Michele TintiCarol Mackintosh
Apr 17, 2014·Chemical Reviews·Lech-Gustav MilroyChristian Ottmann
Dec 9, 2014·Nature Genetics·Martin ReinckeMasayuki Komada
May 26, 2015·Lancet·André LacroixLynnette K Nieman
Jul 26, 2015·Journal of Endocrinological Investigation·L G Perez-Rivas, M Reincke
Sep 9, 2015·Neurosurgery·William F ChandlerDavid E Schteingart
Sep 29, 2015·Trends in Endocrinology and Metabolism : TEM·Silviu SbieraBruno Allolio
Oct 13, 2015·Drug Discovery Today·Yasaman Aghazadeh, Vassilios Papadopoulos
Nov 6, 2015·Angewandte Chemie·Lech-Gustav MilroyChristian Ottmann
Mar 14, 2017·Nature Chemical Biology·Morgan S GaddAlessio Ciulli
May 4, 2017·Current Medicinal Chemistry·Ylenia CauMaurizio Botta
May 16, 2017·The Journal of Clinical Endocrinology and Metabolism·Fabio R FauczConstantine A Stratakis
Jul 12, 2017·Expert Opinion on Drug Discovery·Sebastian A AndreiChristian Ottmann
Oct 3, 2017·Journal of Medicinal Chemistry·Loes M SteversChristian Ottmann
Oct 19, 2017·Journal of the American Chemical Society·David BierChristian Ottmann

❮ Previous
Next ❯

Citations

Jan 22, 2020·The FEBS Journal·Dana KalabovaVeronika Obsilova
Jun 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alice BalloneChristian Ottmann
Mar 17, 2021·Nature Communications·Gergo GoglNikolai N Sluchanko
Jun 6, 2020·Journal of the American Chemical Society·Madita WolterChristian Ottmann
Jun 18, 2021·ACS Medicinal Chemistry Letters·Eline SijbesmaChristian Ottmann
Jun 29, 2021·Frontiers in Pharmacology·Yanfeng Wang, Feng Wang
Aug 4, 2021·Acta Crystallographica. Section F, Structural Biology Communications·Lorenzo SoiniChristian Ottmann
Mar 24, 2020·ACS Omega·Madita WolterChristian Ottmann
Aug 25, 2021·Journal of the American Chemical Society·Xavier GuilloryChristian Ottmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Methods in Molecular Biology
Maria M Lopez, George I Makhatadze
Nederlands tijdschrift voor geneeskunde
J W R EVERSE
Nihon rinsho. Japanese journal of clinical medicine
Ryo Nasushita, T Suda
Nihon rinsho. Japanese journal of clinical medicine
Ryo Nasushita
© 2022 Meta ULC. All rights reserved