Biophysical characterization and functional consequences of a slowly inactivating potassium current in neostriatal neurons

Journal of Neurophysiology
L A Gabel, E S Nisenbaum

Abstract

Neostriatal spiny projection neurons can display a pronounced delay in their transition to action potential discharge that is mediated by a slowly developing ramp depolarization. The possible contribution of a slowly inactivating A-type K+ current (IAs) to this delayed excitation was investigated by studying the biophysical and functional properties of IAs using whole cell voltage- and current-clamp recording from acutely isolated neostriatal neurons. Isolation of IAs from other voltage-gated, calcium-independent K+ currents was achieved through selective blockade of IAs with low concentrations (10 microM) of the benzazepine derivative, 6-chloro-7,8-dihydroxy-3-allyl- 1-phenyl-2,3,4,5-tetra-hydro-1H-3-benzazepine (APB; SKF82958) and subsequent current subtraction. Examination of the voltage dependence of activation showed that IAs began to flow at approximately -60 mV in response to depolarization. The voltage dependence of inactivation revealed that approximately 50% of IAs channels were available at the normal resting potential (-80 mV) of these cells, but that only 20% of the channels were available at membrane potentials corresponding to spike threshold (about -40 mV). At these depolarized membrane potentials, the rate of a...Continue Reading

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Citations

Jul 23, 1999·Annals of the New York Academy of Sciences·P O'DonnellA A Grace
Oct 9, 1999·Journal of Neurophysiology·M MartinaD Paré
Sep 5, 2019·Frontiers in Cellular Neuroscience·Rajnish RanjanHenry Markram
Jul 8, 2003·The Journal of Physiology·Séverine MahonStéphane Charpier
Feb 28, 2002·The Journal of Pharmacology and Experimental Therapeutics·Barbara L WaszczakJames R Stellar
Mar 3, 2012·Brain Structure & Function·Tohru KurotaniKathleen S Rockland
Jan 1, 2013·F1000Research·Gabriele Scheler
Oct 28, 2005·Journal of Neurophysiology·Enrico Bracci, Stefano Panzeri
Jan 23, 2013·Journal of Pharmacokinetics and Pharmacodynamics·Hugo GeertsRobert Carr
Nov 10, 2020·Journal of Computational Neuroscience·Rahmi Elibol, Neslihan Serap Şengör
May 7, 2002·The Journal of Comparative Neurology·Crista L AdamsonRobin L Davis
May 15, 2002·Hippocampus·James R MoyerThomas H Brown

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