Biophysical characterization of beta-thalassemic red blood cells

Cell Biochemistry and Biophysics
Omar S DesoukySeham M El-Marakby

Abstract

Thalassemia is the world's most common hereditary disease; therefore, more interest has been devoted for the development of the screening procedure of this disease. In beta-thalassemia major, the subject of the current study, impaired biosynthesis of beta-globin leads to accumulation of unpaired alpha-globin chain. The objective of the present study, was to examine many of the biophysical properties of beta-thalassemia major red blood cells (RBCs) and to study the possibility of use of any of them as a preliminary screening tool for beta-thalassemia. The percentage of normal hemolysis, osmotic fragility test, turbidity test, rheological properties, and dielectric properties, were studied in 20 regularly blood transfused thalassemia major patients who were under chelation therapy and their status were compared with those of 10 healthy subjects. There was an increase in the percentage of hemolysis for beta-thalassemia by 114.6% compared to the normal RBCs. The fragility curve for beta-thalassemia RBCs showed a shift toward lower NaCl concentration compared to the normal curve. The average osmotic fragility (H(50): the NaCl concentration producing 50% homolysis) for beta-thalassemia was found to be 3.21 +/- 0.67 g/l, whereas for n...Continue Reading

References

Dec 19, 1985·Biochimica Et Biophysica Acta·D Lichtenberg
Feb 1, 1980·British Journal of Haematology·J L AndertonJ Dawes
Jul 6, 1981·Biochimica Et Biophysica Acta·K Araki, J M Rifkind
Mar 26, 1983·British Medical Journal·E Silvestroni, I Bianco
Feb 15, 1980·Biochimica Et Biophysica Acta·I KahaneE A Rachmilewitz
May 1, 1997·Medical & Biological Engineering & Computing·O G MartinsenO Sveen
May 1, 1997·Meditsinskaia tekhnika·N A Dobrovol'skiĭA V Peshkov
Aug 28, 1998·Bioscience, Biotechnology, and Biochemistry·S Iwamoto, H Kumagai
Jun 19, 2003·Physiological Measurement·Dieter HaemmerichJohn G Webster
Apr 1, 1956·Journal of Cellular Physiology·J F HOFFMANA K PARPART
Nov 15, 2003·Biosensors & Bioelectronics·J-H ParkK-Y Ham
Aug 25, 2004·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S SirichotiyakulT Tongsong
May 11, 2005·Journal of Human Nutrition and Dietetics : the Official Journal of the British Dietetic Association·C E GoodchildT Parkin
Oct 31, 2008·Talanta·Supaporn Kradtap HartwellKate Grudpan

Citations

Apr 5, 2011·Physica Medica : PM : an International Journal Devoted to the Applications of Physics to Medicine and Biology : Official Journal of the Italian Association of Biomedical Physics (AIFB)·Mohsen M Mady, Mousa A Allam
May 31, 2013·Clinical Hemorheology and Microcirculation·Hazal Tancer-ElciHülya Aybek
Oct 1, 2017·International Journal of Laboratory Hematology·A Loniewska-LwowskaJ Fabijanska-Mitek
Sep 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yvette Y YienBarry H Paw

Related Concepts

Red Cell Ghost
Erythrocytes
Hemolysis
Osmotic Fragility Test
Flowmetry
Tensides
Viscosity
Thalassemia Minor
Beta-Globins
Biophysical Phenomena

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

© 2021 Meta ULC. All rights reserved