Biophysical properties of dermal building-blocks affects extra cellular matrix assembly in 3D endogenous macrotissue

Biofabrication
Francesco UrciuoloPaolo A Netti

Abstract

The fabrication of functional tissue units is one of the major challenges in tissue engineering due to their in vitro use in tissue-on-chip systems, as well as in modular tissue engineering for the construction of macrotissue analogs. In this work, we aim to engineer dermal tissue micromodules obtained by culturing human dermal fibroblasts into porous gelatine microscaffold. We proved that such stromal cells coupled with gelatine microscaffolds are able to synthesize and to assemble an endogenous extracellular matrix (ECM) resulting in tissue micromodules, which evolve their biophysical features over the time. In particular, we found a time-dependent variation of oxygen consumption kinetic parameters, of newly formed ECM stiffness and of micromodules self-aggregation properties. As consequence when used as building blocks to fabricate larger tissues, the initial tissue micromodules state strongly affects the ECM organization and maturation in the final macrotissue. Such results highlight the role of the micromodules properties in controlling the formation of three-dimensional macrotissue in vitro, defining an innovative design criterion for selecting tissue-building blocks for modular tissue engineering.

References

Jun 5, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Siamak Tabibzadeh
Dec 24, 2002·Biophysical Journal·Yiider TsengDenis Wirtz
May 14, 2003·Trends in Cell Biology·Frederick Grinnell
Aug 21, 2007·Biomaterials·Ali Khademhosseini, Robert Langer
Dec 11, 2007·Biophysical Journal·Christopher B RaubSteven C George
Jun 28, 2008·Lab on a Chip·Priyadarshi PandaPatrick S Doyle
Nov 18, 2008·Biotechnology and Bioengineering·Adam P RagoJeffrey R Morgan
Sep 5, 2009·Journal of Molecular and Cellular Cardiology·Stephanie L K BowersTroy A Baudino
Jan 28, 2010·Acta Biomaterialia·Carmela PalmieroPaolo Netti
Feb 25, 2010·Soft Matter·Jason W Nichol, Ali Khademhosseini
Sep 3, 2010·Biofabrication·Karoly JakabGabor Forgacs
Feb 18, 2011·Disease Models & Mechanisms·Thomas R Cox, Janine T Erler
Mar 2, 2011·Advanced Materials·Yukiko T MatsunagaShoji Takeuchi
Apr 2, 2011·Nature·Monya Baker
Mar 14, 2012·Advanced Materials·Pinar ZorlutunaAli Khademhosseini
Apr 3, 2012·Small·Diego VelascoEugenia Kumacheva
May 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Geeta MehtaShuichi Takayama
Oct 17, 2012·Trends in Cell Biology·Jennifer S Liu, Zev J Gartner
Dec 27, 2012·IEEE Reviews in Biomedical Engineering·Pinar ZorlutunaAli Khademhosseini
Apr 2, 2013·Nature Materials·Hiroaki OnoeShoji Takeuchi
May 25, 2013·Annual Review of Biomedical Engineering·Kyriacos A AthanasiouJerry C Hu
Nov 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nimalan ThavandiranPeter W Zandstra
Apr 24, 2014·Development·Nikolche GjorevskiMatthias P Lutolf
Jul 23, 2014·Biofabrication·Riccardo LevatoMiguel A Mateos-Timoneda
Feb 20, 2015·Journal of Tissue Engineering and Regenerative Medicine·Jeremiah J Riesberg, Wei Shen
Apr 2, 2015·American Journal of Respiratory and Critical Care Medicine·Scott T Weiss
Oct 27, 2015·Biotechnology and Bioengineering·Dilip ThomasDimitrios I Zeugolis

❮ Previous
Next ❯

Citations

Mar 10, 2016·Journal of Nanobiotechnology·Sten Friberg, Andreas M Nyström
Dec 27, 2011·International Journal of Pharmaceutics·Mako KatoYoshie Maitani
Apr 14, 2017·Advanced Healthcare Materials·Bernadette LombardiPaolo Antonio Netti
Dec 19, 2017·Biotechnology and Bioengineering·Vincenza De GregorioPaolo A Netti
Jun 15, 2019·Cellular and Molecular Life Sciences : CMLS·Venkat Raghavan KrishnaswamyIrit Sagi
Dec 8, 2016·Advanced Healthcare Materials·Filomena GioiellaPaolo A Netti
Oct 27, 2016·Advanced Healthcare Materials·Antonio PacielloPaolo A Netti
Oct 17, 2017·Advanced Healthcare Materials·Samad AhadianMilica Radisic
Dec 16, 2018·Biotechnology and Bioengineering·Brunella CorradoPaolo A Netti
Apr 13, 2021·Advanced Functional Materials·Alexander S CaldwellKristi S Anseth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.