Biophysical regulation of Chlamydia pneumoniae-infected monocyte recruitment to atherosclerotic foci

Scientific Reports
Shankar J Evani, Anand K Ramasubramanian

Abstract

Chlamydia pneumoniae infection is implicated in atherosclerosis although the contributory mechanisms are poorly understood. We hypothesize that C. pneumoniae infection favors the recruitment of monocytes to atherosclerotic foci by altering monocyte biophysics. Primary, fresh human monocytes were infected with C. pneumoniae for 8 h, and the interactions between monocytes and E-selectin or aortic endothelium under flow were characterized by video microscopy and image analysis. The distribution of membrane lipid rafts and adhesion receptors were analyzed by imaging flow cytometry. Infected cells rolled on E-selectin and endothelial surfaces, and this rolling was slower, steady and uniform compared to uninfected cells. Infection decreases cholesterol levels, increases membrane fluidity, disrupts lipid rafts, and redistributes CD44, which is the primary mediator of rolling interactions. Together, these changes translate to higher firm adhesion of infected monocytes on endothelium, which is enhanced in the presence of LDL. Uninfected monocytes treated with LDL or left untreated were used as baseline control. Our results demonstrate that the membrane biophysical changes due to infection and hyperlipidemia are one of the key mechanisms...Continue Reading

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Citations

May 23, 2017·Frontiers in Cellular and Infection Microbiology·Dhritiman SamantaStacey D Gilk
Nov 5, 2020·International Journal of Molecular Sciences·Priya VeluswamyJens Wippermann
Dec 16, 2017·Cellular Immunology·Amit K SahaAnand K Ramasubramanian
Jun 10, 2021·Communications Biology·Shreya S DeshmukhUtkan Demirci

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Flow Jo
Image stream
ImagePro
Image Pro Analyzer
GraphPad Prism

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