Biophysical studies on the RNA cores of satellite tobacco mosaic virus

Biophysical Journal
J DayA McPherson

Abstract

Satellite tobacco mosaic virus (STMV) was probed using a variety of proteases. Consequences of the degradation were analyzed using gel electrophoresis, quasi-elastic light scattering (QELS), and atomic force microscopy (AFM). Proteolysis rates of 30 minutes for complete degradation of the protein capsid, up to many hours, were investigated. With each protease, degradation of virions 17 nm in diameter was shown by QELS to result in particles of 10 nm diameter, which is that of the RNA core observed in the virion by x-ray diffraction analysis. This was verified by direct visualization with atomic force microscopy. Using QELS, it was further shown that freshly prepared RNA cores remain as individual, stable, 10-nm condensed particles for 12 to 24 h. Clusters of particles then formed, followed by very large aggregates of 500 to 1000 nm diameter. AFM showed that the aggregates were composed of groups of the condensed RNA cores and were not due to unfolding of the nucleic acid. No unfolding of the core particles into extended conformation was seen by AFM until the samples were heated well beyond 90 degrees C. Mass spectrometry of RNA core particles revealed the presence of a major polypeptide whose amino acid sequence corresponded to...Continue Reading

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Citations

Aug 10, 2010·Nucleic Acids Research·Yuri G KuznetsovAlexander McPherson
Mar 24, 2006·The European Physical Journal. E, Soft Matter·R F Bruinsma
Feb 2, 2011·The Journal of Chemical Physics·A SingharoyP Ortoleva
May 1, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Evgeniy V DubrovinIgor V Yaminsky
Aug 2, 2011·Progress in Biophysics and Molecular Biology·Harshad JoshiPeter J Ortoleva
Mar 15, 2006·Structure·Peter L FreddolinoKlaus Schulten
Jun 1, 2001·Virology·Y G KuznetsovA McPherson
May 9, 2006·Virology·D L MakinoA McPherson
Sep 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·Steven B LarsonAlexander McPherson
Apr 15, 2016·Physical Review. E·R F BruinsmaA Y Grosberg
Dec 13, 2005·Journal of Cell Science·Victor ShahinJoachim E Kühn
Feb 24, 2012·The Journal of Physical Chemistry. B·Abhishek SingharoyPeter J Ortoleva

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