BioPlex 2200 multiplexed system: simultaneous detection of anti-dsDNA and anti-chromatin antibodies in patients with systemic lupus erythematosus

Autoimmunity
Nathalie BardinMarielle Sanmarco

Abstract

Antibodies for double-stranded DNA (anti-dsDNA) and chromatin represent specific markers of systemic lupus erythematosus (SLE). (1) To evaluate the analytical performance of a multiplexed bead assay (BioPlex 2200) for the simultaneous detection of anti-dsDNA and anti-chromatin antibodies, (2) to compare the results for anti-dsDNA with those obtained using Farr assay, and (3) to analyze the clinical relevance of these antibodies when applied to the follow-up of SLE patients with active nephritis. Hundred and five clinically characterized SLE patients and 96 healthy blood donors sera were analyzed by BioPlex 2200. Prevalence of these antibodies was significantly higher (p < 0.0001) in SLE patients than in controls (68 and 70% for anti-dsDNA and anti-chromatin, vs. 1% for both anti-dsDNA and anti-chromatin, respectively). If you consider a sample positive if either anti-dsDNA and/or anti-chromatin is positive, then the prevalence of these antibodies reached 78% (82/105) in SLE patients. For anti-dsDNA measurements, the kappa coefficient was 0.59 between BioPlex 2200 and Farr assay. Comparison between SLE patients with and without nephritis in a follow-up study showed that patients with active nephritis were associated with an incr...Continue Reading

References

Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
May 8, 1998·The New England Journal of Medicine·B H Hahn
Jan 14, 2004·Kidney International·Jan J WeeningUNKNOWN Renal Pathology Society Working Group on the Classification of Lupus Nephritis
Apr 5, 2005·Autoimmunity·Piersandro RiboldiPier Luigi Meroni
Jul 30, 2005·Clinical & Developmental Immunology·Gisele Zandman-GoddardYehuda Shoenfeld
Feb 15, 2007·Nature Clinical Practice. Rheumatology·Michael Mahler, Marvin J Fritzler
May 15, 2007·Rheumatology·D A IsenbergA Rahman
Sep 6, 2007·Annals of the New York Academy of Sciences·Sophie Desplat-JegoMarielle Sanmarco
Sep 6, 2007·Annals of the New York Academy of Sciences·Nurşen DüzgünHüseyin Tutkak
Dec 14, 2007·Autoimmunity·Bahram NamjouJohn B Harley
Jan 12, 2008·The American Journal of Pathology·Elin S MortensenOle P Rekvig

❮ Previous
Next ❯

Citations

May 8, 2014·Journal of Immunology Research·Sonal Mehra, Marvin J Fritzler
Oct 20, 2009·Journal of Immunological Methods·John G HanlyKathleen Wilton
Aug 6, 2019·Frontiers in Immunology·Xiaoyu Wang, Yumin Xia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.