Bioprinting of mineralized constructs utilizing multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink

Biofabrication
Tilman AhlfeldMichael Gelinsky

Abstract

Due to their characteristic resemblance of the mineral component of bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on the development of pasty calcium phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion-based additive manufacturing, namely 3D plotting. While it could be demonstrated that sensitive substances like growth factors can be integrated in such printed CPC scaffolds without impairment of their biological activity live cells cannot be suspended in CPC as they may not be functional when enclosed in a solid and stiff matrix. In contrast, 3D bioprinting of soft cell-laden hydrogels (bioinks) enables the fabrication of constructs with spatially defined cell distribution, which has the potential to overcome problems of conventional cell seeding techniques-but such objects lack mechanical stability. Herein, we combine 3D plotting of CPC and bioprinting of a cell-laden bioink for the first time. As model bioink, an alginate-methylcellulose blend (alg/mc) was used, previously developed by us. Firstly, a fabrication regime was established, enabling optimal setting of CPC and cell survival inside the bioink. As th...Continue Reading

References

Sep 16, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·U HempelK W Wenzel
May 26, 2006·Acta Biomaterialia·Pedro MirandaAntoni P Tomsia
Mar 6, 2008·Journal of Cellular and Molecular Medicine·Wolfgang BöckerMatthias Schieker
Sep 26, 2008·Chemical Reviews·Lijun Wang, George H Nancollas
Jul 25, 2009·Journal of the Mechanical Behavior of Biomedical Materials·Jacqueline M CordellAmy J Wagoner Johnson
Feb 9, 2012·Advanced Drug Delivery Reviews·Maria-Pau GinebraEdgar B Montufar
Apr 3, 2012·Clinical Biochemistry·Jasreen KularJiake Xu
Aug 31, 2012·Journal of Tissue Engineering and Regenerative Medicine·Anja LodeMichael Gelinsky
Jan 26, 2013·Journal of Tissue Engineering and Regenerative Medicine·Joydip KunduDong-Woo Cho
Sep 17, 2013·Advanced Materials·Jos MaldaDietmar W Hutmacher
Feb 11, 2014·Journal of Biomedical Materials Research. Part a·Joshua P TempleWarren L Grayson
Jun 13, 2014·Tissue Engineering. Part C, Methods·Anne BernhardtMichael Gelinsky
Dec 19, 2014·Science Translational Medicine·Jungil ChoiSunghoon Kwon
Feb 3, 2015·Angewandte Chemie·Kristin SchachtThomas Scheibel
Sep 1, 2015·Acta Biomaterialia·Ashwini Rahul AkkineniMichael Gelinsky
Dec 10, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Elisa BoaniniAdriana Bigi
Feb 16, 2016·Nature Biotechnology·Hyun-Wook KangAnthony Atala
Jun 10, 2016·Advanced Healthcare Materials·Andrew C DalyDaniel J Kelly
Oct 8, 2016·Nanoscale·T W LuP T Lee
Nov 23, 2016·Acta Biomaterialia·Yassine MaazouzMaria-Pau Ginebra
Dec 28, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Xin JingLih-Sheng Turng
Apr 19, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Yang SongHockin H K Xu
Apr 21, 2017·Frontiers in Bioengineering and Biotechnology·Shen Ji, Murat Guvendiren
Oct 22, 2017·International Journal of Biological Macromolecules·JiUn LeeGeun Hyung Kim
Nov 9, 2017·ACS Applied Materials & Interfaces·Albert BarbaMaria-Pau Ginebra
Sep 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yongxiang LuoMichael Gelinsky
Apr 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ferry P W MelchelsJos Malda

❮ Previous
Next ❯

Citations

Feb 12, 2020·Advanced Materials·Riccardo LevatoJos Malda
Feb 23, 2020·The Journal of Bone and Joint Surgery. American Volume·Pedro K BeredjiklianMichael Rivlin
Aug 1, 2020·Small·Amin GhavamiNejadAli Khademhosseini
Aug 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Carmen C Piras, David K Smith
Feb 26, 2020·International Journal of Molecular Sciences·Henriette BretschneiderCorina Vater
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Enrique Mancha SánchezJ Blas Pagador
Apr 10, 2020·Frontiers in Bioengineering and Biotechnology·Paula KornMichael Gelinsky
Apr 9, 2020·Frontiers in Pharmacology·Daoyang FanZhongjun Liu
Oct 9, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·S SwethaN Selvamurugan
Jan 29, 2021·ACS Biomaterials Science & Engineering·Janina SpangenbergMichael Gelinsky
Feb 23, 2021·Frontiers in Bioengineering and Biotechnology·Na LiZhenyu Jason Zhang
Apr 21, 2021·Advanced Healthcare Materials·Moyuan QuAli Khademhosseini
May 3, 2021·International Journal of Biological Macromolecules·Balaji MahendiranGopal Shankar Krishnakumar
Feb 25, 2020·ACS Applied Materials & Interfaces·Tilman AhlfeldMichael Gelinsky
May 1, 2020·ACS Applied Materials & Interfaces·Nileshkumar DubeyMarco C Bottino
May 30, 2020·Chemical Reviews·Matthew L BedellAntonios G Mikos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.