Bioprocess Optimisation for High Cell Density Endoinulinase Production from Recombinant Aspergillus niger.

Applied Biochemistry and Biotechnology
Pfariso MaumelaJohann Gorgens

Abstract

Endoinulinase gene was expressed in recombinant Aspergillus niger for selective and high-level expression using an exponential fed-batch fermentation. The effects of the growth rate (μ), glucose feed concentration, nitrogen concentration and fungal morphology on enzyme production were evaluated. A recombinant endoinulinase with a molecular weight of 66 kDa was secreted. Endoinulinase production was growth associated at μ> 0.04 h-1, which is characteristic of the constitutive gpd promoter used for the enzyme production. The highest volumetric activity (670 U/ml) was achieved at a growth rate of 93% of μmax (0.07 h-1), while enzyme activity (506 U/ml) and biomass substrate yield (0.043 gbiomassDW/gglucose) significantly decreased at low μ (0.04 h-1). Increasing the feed concentration resulted in high biomass concentrations and viscosity, which necessitated high agitation to enhance the mixing efficiency and oxygen. However, the high agitation and low DO levels (ca. 8% of saturation) led to pellet disruption and growth in dispersed morphology. Enzyme production profiles, product (Yp/s) and biomass (Yx/s) yield coefficients were not affected by feed concentration and morphological change. The gradual increase in the concentration o...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·P J Punt, C A van den Hondel
Jun 29, 2002·The British Journal of Nutrition·A Franck
May 27, 2004·Bioprocess and Biosystems Engineering·Beom Soo KimHo Nam Chang
Jan 18, 2005·Journal of Biotechnology·J L Casas LópezY Chisti
Sep 1, 2005·Applied Microbiology and Biotechnology·Martin B HaackAnna Eliasson Lantz
Jun 20, 2008·Microbial Cell Factories·Simon TännlerUwe Sauer
Mar 3, 2009·Applied and Environmental Microbiology·Björn AlrikssonLeif J Jönsson
Apr 15, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Andreas Wargenau, Arno Kwade
May 27, 2010·Applied Microbiology and Biotechnology·Habib DriouchChristoph Wittmann
Jul 10, 2012·Journal of Biotechnology·Rainer KrullChristoph Wittmann
Mar 23, 2015·Advances in Biochemical Engineering/biotechnology·Robert WaliskoRainer Krull
Apr 29, 2015·International Journal of Molecular Sciences·Eva JohanssonHelena Persson Hovmalm
Aug 15, 2015·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Paula Monteiro de SouzaPérola Oliveira Magalhães
Sep 19, 2015·Critical Reviews in Biotechnology·Jianguo Zhang, Jining Zhang
Mar 11, 2016·Journal of Agricultural and Food Chemistry·Yanbing XuJia Ouyang
Dec 13, 2014·Biotechnology Reports·Linxi YangChibuike C Udenigwe
Feb 24, 2018·Applied Microbiology and Biotechnology·Lukas VeiterChristoph Herwig

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