Bioprocess optimization of PHB homopolymer and copolymer P3 (HB-co-HV) by Acinetobacter junii BP25 utilizing rice mill effluent as sustainable substrate

Environmental Technology
Poorna Chandrika SabapathyPreethi Kathirvel

Abstract

The potential use of parboiled rice mill effluent as a cheap substrate for the production of homopolymer and copolymer of Polyhydroxyalkanoates (PHAs) by Acinetobacter junii BP 25 was investigated for the first time. Process optimization by one factor at a time led to homopolymer polyhydroxybutyrate (PHB) production of 2.64 ± 0.18 g/l with 94.28% PHB content using a two-stage batch cultivation mode. BP 25 furthermore produced polyhydroxybutyrate-co-hydroxyvalerate (P3 (HB-co-HV)), with the addition of valeric acid as an additive to the substrate, yielding (2.56 ± 0.12 g/l dry biomass, 2.20 ± 0.15 g/l PHA) a copolymer content of 85.93%. Thus, rice mill effluent can be an effective and relatively low-cost alternative for the production of PHA, replacing the pure substrates.

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Methods Mentioned

BETA
PCR

Software Mentioned

BLAST ( Basic Local Alignment Search Tool )
Molecular Evolutionary Genetics Analysis ( MEGA )

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