Bioproduction of benzaldehyde in a solid-liquid two-phase partitioning bioreactor using Pichia pastoris

Biotechnology Letters
Ashu N JainAndrew J Daugulis

Abstract

The bioproduction of benzaldehyde from benzyl alcohol using Pichia pastoris was examined in a solid-liquid two-phase partitioning bioreactor (TPPB) to reduce substrate and product inhibition. Rational polymer selection identified Elvax 40W as an effective sequestering phase, possessing partition coefficients for benzyl alcohol and benzaldehyde of 3.5 and 35.4, respectively. The use of Elvax 40W increased the overall mass of benzaldehyde produced by approx. 300% in a 5 l bioreactor, relative to a single phase biotransformation. The two-phase system had a molar yield of 0.99, indicating that only minor losses occurred. These results provide a promising starting point for solid-liquid TPPBs to enhance benzaldehyde production, and suggest that multiple, targeted polymers may provide relief for transformations characterized by multiple inhibitory substrates/product/by-products.

References

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Citations

Jun 4, 2013·Applied and Environmental Microbiology·Richard VillemurEric Déziel
Jul 30, 2014·Biotechnology Advances·Wouter Van HeckeHeleen De Wever
Jul 5, 2011·Metabolic Engineering·Rebekah McKenna, David R Nielsen
Jul 19, 2014·Bioprocess and Biosystems Engineering·Rebekah McKennaDavid R Nielsen
Jan 13, 2015·Applied and Environmental Microbiology·Aditya M Kunjapur, Kristala L J Prather
Jul 31, 2014·Journal of the American Chemical Society·Aditya M KunjapurKristala L J Prather
Dec 1, 2021·Environmental Science and Pollution Research International·Shi ShiYanli Zhou

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