Bioprospecting for Antibacterial Drugs: a Multidisciplinary Perspective on Natural Product Source Material, Bioassay Selection and Avoidable Pitfalls

Pharmaceutical Research
T P Tim CushnieSimon M Clow

Abstract

Bioprospecting is the exploration, extraction and screening of biological material and sometimes indigenous knowledge to discover and develop new drugs and other products. Most antibiotics in current clinical use (eg. β-lactams, aminoglycosides, tetracyclines, macrolides) were discovered using this approach, and there are strong arguments to reprioritize bioprospecting over other strategies in the search for new antibacterial drugs. Academic institutions should be well positioned to lead the early stages of these efforts given their many thousands of locations globally and because they are not constrained by the same commercial considerations as industry. University groups can lack the full complement of knowledge and skills needed though (eg. how to tailor screening strategy to biological source material). In this article, we review three key aspects of the bioprospecting literature (source material and in vitro antibacterial and toxicity testing) and present an integrated multidisciplinary perspective on (a) source material selection, (b) legal, taxonomic and other issues related to source material, (c) cultivation methods, (d) bioassay selection, (e) technical standards available, (f) extract/compound dissolution, (g) use of...Continue Reading

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Feb 1, 1985·Toxicology Letters·E Borenfreund, J A Puerner
Dec 1, 1968·Applied Microbiology·H Basch, H H Gadebusch
Oct 1, 1984·Cell Biology and Toxicology·E Borenfreund, O Borrero
Jan 1, 1997·Toxicologic Pathology·W H Halliwell
Dec 13, 2000·Mutation Research·K Mortelmans, E Zeiger
May 1, 2002·Nature Biotechnology·Joseph A DeVitoDavid L Pompliano
Apr 19, 2003·The Journal of Antimicrobial Chemotherapy·Monique I Andersson, Alasdair P MacGowan
Apr 26, 2003·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C Rodríguez-BurfordW E Grizzle
May 16, 2003·International Microbiology : the Official Journal of the Spanish Society for Microbiology·David Smith
Aug 25, 2004·Journal of Ethnopharmacology·C W FennellJ van Staden
Jun 21, 2005·Journal of Ethnopharmacology·J L Ríos, M C Recio
Mar 23, 2006·Journal of Industrial Microbiology & Biotechnology·Julian Davies
Oct 17, 2006·The Journal of Antimicrobial Chemotherapy·G L French
Dec 13, 2006·Nature Biotechnology·Kim Lewis, Frederick M Ausubel
Feb 24, 2007·Journal of Ethnopharmacology·P J HoughtonG Steventon
Feb 6, 2008·Antimicrobial Agents and Chemotherapy·Cassie F PopeStephen H Gillespie
Apr 11, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ming-Hsuang ChoMenghang Xia
Jun 28, 2008·Journal of Ethnopharmacology·S F van Vuuren
Jul 5, 2008·Nature Protocols·Guillermo RepettoJorge L Zurita
Jul 11, 2009·Science·Jesse W-H Li, John C Vederas
Aug 4, 2009·Nature Reviews. Drug Discovery
Oct 21, 2009·British Journal of Biomedical Science·J E MayC Donaldson
Jan 30, 2010·International Journal of Molecular Sciences·Rocío González-LamotheKamal Bouarab
May 18, 2010·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Katalin Molnár, Edit Farkas
Sep 17, 2010·Chemistry : a European Journal·Chambers C Hughes, William Fenical
Oct 16, 2010·Methods in Molecular Biology·Rosamaria Tedeschi, Paolo De Paoli
Jan 15, 2011·Clinical Microbiology Reviews·Lynn L Silver
Mar 2, 2011·Future Medicinal Chemistry·Pavan K VaddadyBernd Meibohm
Apr 7, 2011·Methods in Molecular Biology·Terry L RissAndrew L Niles
Apr 26, 2011·International Journal of Antimicrobial Agents·T P Tim Cushnie, Andrew J Lamb
May 31, 2011·Critical Reviews in Biotechnology·Kavita Tiwari, Rajinder K Gupta
Jun 11, 2011·Insect Biochemistry and Molecular Biology·Norman A RatcliffePatricia Azambuja
Jun 29, 2011·ILAR Journal·John E Cooper
Jun 30, 2011·Frontiers in Pharmacology·Erwin L Roggen
Jan 1, 2010·Frontiers in Pharmacology·Olavi Pelkonen
Aug 9, 2011·Expert Review of Anti-infective Therapy·Sheo B SinghLynn Miesel
Jan 21, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mathieu VinkenVera Rogiers
May 18, 2012·FEMS Microbiology Letters·Angharad E GreenSarah E Maddocks

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Citations

Jan 13, 2021·Chemistry : a European Journal·Cédric TresseStéphanie Norsikian
Jan 16, 2021·Angewandte Chemie·Bart HerléAlois Fürstner
Aug 3, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Zhijun MiaoRajeev K Singla

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Methods Mentioned

BETA
light microscopy
Assay
ELISA
protease assay

Software Mentioned

Riss
Derek Nexus

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