Bioprospection of Basidiomycetes and molecular phylogenetic analysis using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence

Scientific Reports
Thangamalai Mowna SundariRajaiah Shenbagarathai

Abstract

Macrofungi belonging to the phylum Basidiomycota are mostly used as medicinal mushrooms in many countries. In the present study, hundred basidiocarp of macrofungi were collected from Tamilnadu during rainy season. The basidiocarp was found in association with root/trunk of living trees, wood log and decayed matter. Among the hundred basidiocarp, 49 were grown into axenic cultures. Notable variations in the macroscopic characteristics of the basidiome and culture morphology were observed. To study the genetic diversity, the molecular taxonomy of the isolates was carried out using internal transcribed spacer (ITS) and 5.8S rRNA gene sequence marker. Thirty-two strains belonging to the order Polyporales, Hymenochataeles and Russuales under the division Basidiomycota were classified based on phylogeny analysis. This study provides first evidence for the occurrence of species Fulvifomes fastuosus (LDCMY39 and LDCMY43) and Ganoderma wiiroense (LDCMY02, LDCMY08, LDCMY11, LDCMY17 and LDCMY19) from southern India. Molecular evidence for the existence of Phellinus badius was given for the first time as well. These data enhance our understanding on the diversity of macrofungi in India, which could be further exploited for biomedical appli...Continue Reading

References

Mar 1, 1992·The Australian and New Zealand Journal of Psychiatry·P D McGorry
Dec 1, 1991·The Quarterly Review of Biology·D M Hillis, M T Dixon
Jul 10, 1999·Clinical Microbiology Reviews·J GuarroA M Stchigel
Nov 19, 2002·Applied Microbiology and Biotechnology·S P Wasser
Mar 8, 2005·Mycopathologia·K V SankaranC Gokulapalan
Aug 2, 2005·FEMS Immunology and Medical Microbiology·Emilie FréalleLaurence Delhaes
Mar 18, 2008·Applied and Environmental Microbiology·Andrea Porras-AlfaroDonald O Natvig
May 23, 2009·FEMS Microbiology Letters·Tony Vancov, Brad Keen
May 1, 2009·Molecular Ecology Resources·Keith A Seifert
Jul 16, 2011·European Journal of Clinical Nutrition·I HuybrechtsUNKNOWN EFCOVAL Consortium
Mar 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Conrad L SchochUNKNOWN Fungal Barcoding Consortium Author List
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 17, 2015·Bioorganic & Medicinal Chemistry Letters·Byung Soon HwangBong-Sik Yun
Jan 30, 2016·Persoonia·P W CrousJ Z Groenewald

❮ Previous
Next ❯

Datasets Mentioned

BETA
FJ982798

Methods Mentioned

BETA
PCR

Software Mentioned

DNA Baser
MEGA6
MEGA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.