Bioprospection of cytotoxic compounds in fungal strains recovered from sediments of the Brazilian coast

Chemistry & Biodiversity
Bárbara S F RodriguesMaria C F Oliveira

Abstract

The cytotoxic activities of extracts (50 μg/ml) from 48 fungal strains, recovered from sediments of Pecém's offshore port terminal (Northeast coast of Brazil), against HCT-116 colon cancer cell lines were investigated. The most promising extract was obtained from strain BRF082, identified as Dichotomomyces cejpii by phylogenetic analyses of partial RPB2 gene sequence. Thus, it was selected for bioassay-guided isolation of the cytotoxic compounds. Large-scale fermentation of BRF082 in potato dextrose broth, followed by chromatographic purification of the bioactive fractions from the liquid medium, yielded gliotoxin (4) and its derivatives acetylgliotoxin G (3), bis(dethio)bis(methylsulfanyl)gliotoxin (1), acetylgliotoxin (5), 6-acetylbis(dethio)bis(methylsulfanyl)gliotoxin (2), besides the quinazolinone alkaloid fiscalin B. All isolated compounds were tested for their cytotoxicities against the tumor cell lines HCT-116, revealing 4 and 3 as the most cytotoxic ones (IC50 0.41 and 1.06 μg/ml, resp.).

References

Nov 1, 1992·The Journal of Antibiotics·D Van der PylS Omura
Jan 1, 1965·Chemotherapy·N M LarinR B Wenham
Jan 1, 1969·Archiv für die gesamte Virusforschung·J K McDougall
Dec 1, 1981·The Journal of Antibiotics·N Kitahara, A Endo
Jun 1, 1994·International Journal of Food Microbiology·E PieckováZ Jesenská
Aug 1, 1993·International Journal of Food Microbiology·Z JesenskáD Bernát
May 20, 1997·International Journal of Food Microbiology·E Piecková, Z Jesenská
Dec 22, 1999·Molecular Biology and Evolution·Y J LiuB D Hall
May 24, 2000·Mycopathologia·E Piecková, H Roeijmans
Oct 24, 2001·The Journal of Organic Chemistry·Haishan Wang, A. Ganesan
Nov 6, 2001·Archives of Pharmacal Research·H J LeeJ J Lee
Dec 26, 1964·Nature·W A RIGHTSELG J DIXON
Jun 11, 2005·Environmental Microbiology·Paul R JensenWilliam Fenical
Nov 17, 2006·Nature Chemical Biology·William Fenical, Paul R Jensen
May 15, 2007·Canadian Journal of Microbiology·Kimberly M MayerJeffrey L C Wright
Dec 1, 2010·Marine Drugs·Ira Bhatnagar, Se-Kwon Kim
Jan 14, 2011·Natural Product Reports·Mostafa E Rateb, Rainer Ebel
Nov 19, 2011·Applied Microbiology and Biotechnology·Daniel H ScharfChristian Hertweck
Feb 7, 2012·Studies in Mycology·J Houbraken, R A Samson
Feb 16, 2012·The Journal of Antibiotics·Masanari TakahashiMinoru Yoshida
Feb 22, 2012·Chemistry & Biodiversity·Yu WangHui-Ming Hua
Oct 20, 2012·Chemistry & Biodiversity·Tasso G C MontenegroLetícia V Costa-Lotufo

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Citations

Jan 19, 2016·Frontiers in Microbiology·Jens C Frisvad, Thomas O Larsen
Aug 2, 2018·Marine Drugs·Solida LongMadalena M M Pinto
Mar 16, 2017·Natural Product Reports·John W BluntMichèle R Prinsep
Feb 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Solida LongMadalena M M Pinto
Nov 4, 2020·Planta medica·Diego V WilkeLeticia V Costa-Lotufo
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Solida LongMadalena M M Pinto

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