Bioprotection Can Be Tuned with a Proper Protein/Saccharide Ratio: The Case of Solid Amorphous Matrices

The Journal of Physical Chemistry. B
Sergio GiuffridaGrazia Cottone

Abstract

Saccharides, and in particular trehalose, are well known for their high efficiency in protecting biostructures against adverse environmental conditions. The protein dynamics is known to be highly inhibited in a low-water trehalose host medium, the inhibition being markedly dependent on the amount of residual water. Besides hydration, the protein/sugar ratio is expected to affect the properties of saccharide amorphous matrices. In this work, we report an infrared spectroscopy study in dry amorphous matrices of various sugars (the disaccharides trehalose, maltose, sucrose, and lactose, and the trisaccharide raffinose) containing myoglobin, at different protein/sugar ratios. We analyze the stretching band of the bound CO molecule and the water association band. Such bands have already been successfully exploited for the simultaneous study of thermal evolution of a matrix and embedded protein. The results show a high dependence of protein and matrix signals on the protein/sugar ratio, the system behavior evolving from situations where (i) the protein slaves the matrix to (ii) protein ↔ matrix coupling/uncoupling, then to (iii) the matrix slaving the protein, with increasing sugar concentration. This supports a mutual protein ↔ matr...Continue Reading

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Citations

Jan 18, 2020·Annual Review of Food Science and Technology·Fernando Luís Barroso da SilvaErik E Santiso
Sep 16, 2020·Physical Chemistry Chemical Physics : PCCP·Christoffer OlssonJan Swenson
Nov 6, 2020·Cell Communication and Signaling : CCS·Cherie Hesgrove, Thomas C Boothby
Apr 4, 2021·International Journal of Molecular Sciences·Sergio GiuffridaGrazia Cottone
Sep 10, 2020·The Journal of Physical Chemistry. B·Lin Wei, Ryo Shirakashi

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