Bioreactor microbial ecosystems with differentiated methanogenic phenol biodegradation and competitive metabolic pathways unraveled with genome-resolved metagenomics

Biotechnology for Biofuels
Feng JuTong Zhang

Abstract

Methanogenic biodegradation of aromatic compounds depends on syntrophic metabolism. However, metabolic enzymes and pathways of uncultured microorganisms and their ecological interactions with methanogenic consortia are unknown because of their resistance to isolation and limited genomic information. Genome-resolved metagenomics approaches were used to reconstruct and dissect 23 prokaryotic genomes from 37 and 20 °C methanogenic phenol-degrading reactors. Comparative genomic evidence suggests that temperature difference leads to the colonization of two distinct cooperative sub-communities that can respire sulfate/sulfite/sulfur or nitrate/nitrite compounds and compete for uptake of methanogenic substrates (e.g., acetate and hydrogen). This competition may differentiate methanogenesis. The uncultured ε-Proteobacterium G1, whose close relatives have broad ecological niches including the deep-sea vents, aquifers, sediment, limestone caves, spring, and anaerobic digesters, is implicated as a Sulfurovum-like facultative anaerobic diazotroph with metabolic versatility and remarkable environmental adaptability. We provide first genomic evidence for butyrate, alcohol, and carbohydrate utilization by a Chloroflexi T78 clade bacterium, an...Continue Reading

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Citations

Jan 16, 2020·Environmental Science and Pollution Research International·Jing WangWei Wang
Feb 26, 2021·Applied Microbiology and Biotechnology·M Concetta TomeiLorenzo Brusetti

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Methods Mentioned

BETA
PCA

Software Mentioned

ChimeraSlayer
MEGA
CLC Genomics Workbench
CLC assembler
Bowtie 2
RNAmmer
BLASTN
PAST3
R
hmmsearch

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