Bioreactor Scalability: Laboratory-Scale Bioreactor Design Influences Performance, Ecology, and Community Physiology in Expanded Granular Sludge Bed Bioreactors

Frontiers in Microbiology
Stephanie ConnellyGavin Collins

Abstract

Studies investigating the feasibility of new, or improved, biotechnologies, such as wastewater treatment digesters, inevitably start with laboratory-scale trials. However, it is rarely determined whether laboratory-scale results reflect full-scale performance or microbial ecology. The Expanded Granular Sludge Bed (EGSB) bioreactor, which is a high-rate anaerobic digester configuration, was used as a model to address that knowledge gap in this study. Two laboratory-scale idealizations of the EGSB-a one-dimensional and a three- dimensional scale-down of a full-scale design-were built and operated in triplicate under near-identical conditions to a full-scale EGSB. The laboratory-scale bioreactors were seeded using biomass obtained from the full-scale bioreactor, and, spent water from the distillation of whisky from maize was applied as substrate at both scales. Over 70 days, bioreactor performance, microbial ecology, and microbial community physiology were monitored at various depths in the sludge-beds using 16S rRNA gene sequencing (V4 region), specific methanogenic activity (SMA) assays, and a range of physical and chemical monitoring methods. SMA assays indicated dominance of the hydrogenotrophic pathway at full-scale whilst a ...Continue Reading

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Citations

Dec 25, 2019·Environmental Science and Pollution Research International·Nishat KhanMohammad ZainKhan
Jan 13, 2019·Environmental Science and Pollution Research International·Yu-Chung LinJustin Chun-Te Lin
Jun 26, 2020·Frontiers in Microbiology·Anna Christine TregoGavin Collins
Dec 11, 2019·Science Progress·Alexander Wy ChanHerb E Schellhorn
Jun 3, 2021·International Journal of Environmental Research and Public Health·Keisy TorresPaula Marzal

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Methods Mentioned

BETA
Assay
PCR
Phyloseq
SMA

Software Mentioned

Vegan
RDPclassifier
) Probe Match tool
PERMANOVA
Phyloseq
adonis
Software
UPARSE
USEARCH
FastTree

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