PMID: 164009Jan 1, 1975

Bioregulation of lysosomal enzyme secretion from human neutrophils: roles of guanosine 3':5'-monophosphate and calcium in stimulus-secretion coupling

Proceedings of the National Academy of Sciences of the United States of America
R J Smith, L J Ignarro

Abstract

The biologic role of calcium and guanosine 3':5'-monophosphate (cyclic GMP) in the immunologic secretion of lysosomal enzymes from human neutrophils was studied. Contact of neutrophils with zymosan-treated serum or the divalent cation ionophore A-23187, in the presence of extracellular calcium, resulted in beta-glucuronidase (beta-D-glucuronide glucuronosohydrolase, EC 3.2.1.31) secretion and a concomitant accumulation of cyclic GMP without any loss of cell viability. Acetylcholine (0.1 muM), in the presence of calcium, enhanced the immunologic stimulation of cyclic GMP accumulation and lysosomal enzyme discharge. A marked and rapid association of 45CaCl2 with neutrophils occurred during cell surface contact with zymosan-treated serum, and this effect on calcium association was enhanced with 0.1 muM acetylcholine. The precise mechanism of the neutrophil-calcium interaction is presently not well understood. However, the finding that 0.5-1.0 muM A-23187 also provoked a rapid association of extracellular calcium with neutrophils suggests that calcium mobilization into the intracellular environment may account, at least in part, for this association between cells and calcium. The close temporal relationship between beta-glucuronida...Continue Reading

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Citations

May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L J Ignarro, W J George
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D E Cochrane, W W Douglas
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Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G SchultzE W Sutherland

Related Concepts

Guanosine
Cations, Divalent
Calcium [EPC]
Neutrophil Band Cells
Extracellular
Calcium
Cyclic GMP Measurement (Lab Test)
A-23187
Protoplasm
Increased Immunologic Activity [PE]

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