Biosafety assessment of conducting nanostructured materials by using co-cultures of neurons and astrocytes

Neurotoxicology
M P LichtensteinC Suñol

Abstract

Neural electrode implants are made mostly of noble materials. We have synthesized a nanostructured material combining the good electrochemical properties of iridium oxide (IrOx) and carbon-nanotubes (CNT) and the properties of poly(3,4-ethylenedioxythiophene) (PEDOT). IrOx-CNT-PEDOT charge storage capacity was lower than that of IrOx and IrOx-CNT, but higher than that of other PEDOT-containing hybrids and Pt. Cyclic voltammetry, SEM, XPS and micro-Raman spectroscopy suggest that PEDOT encapsulates IrOx and CNT. In our search for a cell culture platform that could optimize modelling the in vivo environment, we determined cell viability, neuron and astrocyte functionality and the response of astrocytes to an inflammatory insult by using primary cultures of neurons, of astrocytes and co-cultures of both. The materials tested (based on IrOx, CNT and PEDOT, as well as Pt as a reference) allowed adhesion and proliferation of astrocytes and full compatibility for neurons grown in co-cultures. Functionality assays show that uptake of glutamate in neuron-astrocyte co-culture was significantly higher than the sum of the uptake in astrocytes and neurons. In co-cultures on IrOx, IrOx-CNT and IrOx-CNT-PEDOT, glutamate was released by a depo...Continue Reading

Citations

Jan 16, 2020·Topics in Current Chemistry·Viviana NegriPilar López-Larrubia
Mar 7, 2021·Polymers·I Jénnifer GómezNuria Alegret
Jun 3, 2021·ACS Sensors·Federica MarianiErika Scavetta
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nieves Casañ-Pastor
Jul 2, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiangyun YaoCunyi Fan
Dec 16, 2021·Chemical Reviews·Charalampos PitsalidisRóisín M Owens

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