PMID: 9181299Jun 1, 1997Paper

Biosafety guidelines for sorting of unfixed cells

Cytometry
I SchmidP N Dean

Abstract

The International Society of Analytical Cytology (ISAC) Biohazard Working Group presents guidelines for sorting of unfixed cells, including known biohazardous samples, using jet-in-air, deflected-droplet cell sorters. There is a risk that personnel operating these instruments could become exposed to droplets and aerosols containing biological agents present in the samples. The following guidelines can aid in the prevention of exposures of laboratory personnel to pathogens contained in the sort samples. The document provides biosafety recommendations for sample handling, operator training and protection, laboratory facility design, and instrument setup and maintenance. In addition, it describes in detail methods for assessment of instrument aerosol containment. Recommendations provided here may also help laboratories to obtain institutional and/or regulatory agency approval for sorting of unfixed and known biohazardous samples.

Citations

Mar 3, 2012·Methods : a Companion to Methods in Enzymology·Ingrid Schmid
Sep 24, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Manfred R KollerBernhard Ø Palsson
Sep 16, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·James F Leary
Jun 24, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Klaus LennartzUwe Kirstein
Nov 11, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ingrid SchmidStephen P Perfetto
Mar 28, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ingrid SchmidUNKNOWN International Society of Analytical Cytology
Nov 5, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kevin L Holmes
Mar 19, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kevin L HolmesStephen P Perfetto
Apr 3, 2012·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B KakasiS Nagy
Sep 5, 2008·Current Protocols in Cytometry·I SchmidJ Ferbas
Jan 25, 2012·Cytotechnology·Julien PicotChantal M Boulanger
Sep 5, 2008·Current Protocols in Cytometry·Andrew S Oberyszyn
Sep 5, 2008·Current Protocols in Cytometry·K R Harkins
Sep 5, 2008·Current Protocols in Cytometry·Ingrid SchmidStephen P Perfetto
Jun 4, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kristen M ReifelStephen P Perfetto
Jan 14, 2010·Current Protocols in Cytometry·Larry W Arnold, Joanne Lannigan
May 31, 2008·Analytical Chemistry·Thomas D PerroudKamlesh D Patel

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