Biosafety in handling gene transfer vectors

Current Protocols in Human Genetics
Andrew Braun

Abstract

This unit is devoted to safety issues that must be considered when generating and working with vectors for gene therapy. A short description of the relevant regulatory bodies and mechanisms is followed by a general discussion of containment and safety procedures. This is followed by vector-specific guidelines for nine commonly used vector systems, including adenovirus, vaccinia, HSV-1, lentivirus, and nonviral vectors. Recent tragic experiences with retroviral integration near oncogene loci demonstrate the surprise effects of successful gene therapy. The unit also outlines the problems encountered when taking a promising vector to clinical trials.

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Citations

Jul 21, 2011·Pharmaceutical Research·Man Lung Yeung, Kuan-Teh Jeang
Apr 25, 2009·Journal of Cellular Physiology·Christopher S Gondi, Jasti S Rao
Jan 27, 2021·JMIR MHealth and UHealth·Johnblack K KabukyeRonald Cornet
Mar 25, 2021·Journal of Medical Internet Research·Judith BorghoutsDara H Sorkin
Jul 22, 2021·JMIR Research Protocols·Nelson ShenGillian Strudwick

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