Biosafety of GM Crop Plants Expressing dsRNA: Data Requirements and EU Regulatory Considerations

Frontiers in Plant Science
Salvatore ArpaiaAntje Dietz-Pfeilstetter

Abstract

The use of RNA interference (RNAi) enables the silencing of target genes in plants or plant-dwelling organisms, through the production of double stranded RNA (dsRNA) resulting in altered plant characteristics. Expression of properly synthesized dsRNAs in plants can lead to improved crop quality characteristics or exploit new mechanisms with activity against plant pests and pathogens. Genetically modified (GM) crops exhibiting resistance to viruses or insects via expression of dsRNA have received authorization for cultivation outside Europe. Some products derived from RNAi plants have received a favourable opinion from the European Food Safety Authority (EFSA) for import and processing in the European Union (EU). The authorization process in the EU requires applicants to produce a risk assessment considering food/feed and environmental safety aspects of living organisms or their derived food and feed products. The present paper discusses the main aspects of the safety assessment (comparative assessment, molecular characterization, toxicological assessment, nutritional assessment, gene transfer, interaction with target and non-target organisms) for GM plants expressing dsRNA, according to the guidelines of EFSA. Food/feed safety ...Continue Reading

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Citations

Dec 18, 2020·Frontiers in Physiology·Alexandre Augusto Pereira FirminoMaria Fátima Grossi-de-Sa
Apr 8, 2021·Communications Biology·Jonathan WillowEve Veromann

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Methods Mentioned

BETA
transgenic
genetic modification
targeted modification

Software Mentioned

iPlanta

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