Abstract
Recently, local sustained-release antibiotics systems have been developed because they can increase local foci of concentrated antibiotics without increasing the plasma concentration, and thereby effectively decrease any systemic toxicity and side effects. A vancomycin-loaded bone-like hydroxyapatite/poly-amino acid (V-BHA/PAA) bony scaffold was successfully fabricated with vancomycin-loaded poly lactic-co-glycolic acid microspheres and BHA/PAA, which was demonstrated to exhibit both porosity and perfect biodegradability. The aim of this study was to systematically evaluate the biosafety of this novel scaffold by conducting toxicity tests in vitro and in vivo. According to the ISO rules for medical implant biosafety, for in vitro tests, the scaffold was incubated with L929 fibroblasts or rabbit noncoagulant blood, with simultaneous creation of positive control and negative control groups. The growth condition of L929 cells and hemolytic ratio were respectively evaluated after various incubation periods. For in vivo tests, a chronic osteomyelitis model involving the right proximal tibia of New Zealand white rabbits was established. After bacterial identification, the drug-loaded scaffold, drug-unloaded BHA/PAA, and poly (methyl ...Continue Reading
References
Apr 29, 2005·The Journal of Antimicrobial Chemotherapy·Li-Yan YinJason H Calhoun
Jul 28, 2010·Archives of Orthopaedic and Trauma Surgery·Fei WangJun Liu
Dec 14, 2011·Clinical Pharmacokinetics·Amélie MarsotNicolas Simon
Jul 25, 2012·Drug Delivery·Jingzhe ZhangGuangyao Liu
Oct 30, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ashlee N Ford VersyptRichard D Braatz
Dec 20, 2012·The Journal of Antimicrobial Chemotherapy·S J VandecasteeleE Tacconelli
Mar 26, 2013·Polymer·Jijun HuangAntoni P Tomsia
Apr 19, 2013·Journal of Biomaterials Science. Polymer Edition·Alexia Hernandez-SoriaMathias Bostrom
Jul 16, 2013·The Journal of Craniofacial Surgery·Yoshiaki SakamotoKazuo Kishi
Aug 21, 2013·BioMed Research International·Konstantinos Anagnostakos, Katrin Koch
Oct 1, 2013·Colloids and Surfaces. B, Biointerfaces·Feng QiZhiguo Su
Oct 26, 2013·The Journal of Trauma and Acute Care Surgery·Stephen W DaviesRobert G Sawyer
Nov 6, 2013·Biological & Pharmaceutical Bulletin·Masanori Kikuchi
Nov 29, 2013·The Open Orthopaedics Journal·Gerrit Steffen MaierUwe Maus
Apr 25, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tzong-Yuan JuangLong-Liu Lin
May 24, 2014·International Journal of Nanomedicine·Xingtao ChenJie Wei
May 20, 2015·Acta biochimica Polonica·Tobiasz DeptułaRyszard Słomski
Citations
Aug 17, 2016·Biomaterials Science·Chao HeChang-Sheng Zhao
Feb 9, 2018·PloS One·Yao-Zhong ChenGen-Di Liu
Sep 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chao HeChang-Sheng Zhao
Aug 11, 2021·Pharmaceutics·Manuela MulazziMonica Sandri
Sep 5, 2021·Journal of Biological Engineering·Wenhui ChuZhe Ma