Biosensor Based on Tyrosinase Immobilized on Graphene-Decorated Gold Nanoparticle/Chitosan for Phenolic Detection in Aqueous

Sensors
Fuzi Mohamed FartasMohd Izham Saiman

Abstract

In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.

References

Apr 5, 2008·Biosensors & Bioelectronics·Shengfu WangGuodong Liu
Dec 11, 2008·The Journal of Physical Chemistry. B·B X GuX S Li
Apr 11, 2012·Angewandte Chemie·Mohd Izham bin SaimanGraham J Hutchings
Jul 20, 2012·Biosensors & Bioelectronics·Carmen C Mayorga-MartinezArben Merkoçi
Jul 24, 2015·Biosensors & Bioelectronics·K Kamil RezaA M Biradar

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Citations

Mar 29, 2019·Biotechnology and Applied Biochemistry·Nasim MalekiNahid Shahabadi
Sep 13, 2018·Current Stem Cell Research & Therapy·Shabnam MohebbiMasoud Mozafari
May 28, 2019·Environmental Science and Pollution Research International·Shivamurthy Ravindra YashasNingappa Kumara Swamy
May 23, 2020·Critical Reviews in Biochemistry and Molecular Biology·Mark-Anthony McLarin, Ivanhoe K H Leung
Feb 1, 2020·International Journal of Biological Macromolecules·Congqiang LouZhanhu Guo
Nov 17, 2020·Analytica Chimica Acta·Paulo A Raymundo-PereiraOrlando Fatibello-Filho
Feb 13, 2021·Sensors·Coral Salvo-CominoMaria Luz Rodriguez-Mendez

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Methods Mentioned

BETA
biosensors
biosensor
biosensing
scanning electron microscopy
X-Ray
infrared spectroscopy

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