Biosensor-based selective detection of Zika virus specific antibodies in infected individuals

Biosensors & Bioelectronics
Gustavo Cabral-MirandaMartin F Bachmann

Abstract

Zika virus (ZIKV) recently emerged as a global threat subsequent to its global spread because it induces microencephaly and other brain damages in infants born to infected mothers. Epidemiological monitoring of infection has been hampered by the absence of reliable serological tests capable to distinguish between ZIKV and other Flavivirus infections, in particular Dengue virus (DENV). As both viruses are transmitted by the same mosquito-species, their distributions largely overlap and reliable serological distinction between the viruses is essential. Here we develop a novel biosensor which is based on recombinant forms of ZIKV non-structural protein 1 (NS1) and the domain III of the envelope protein (EDIII). Using electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV), we demonstrate that in addition to extremely sensitive detection of ZIKV-specific antibodies in serum and saliva, the biosensor promptly distinguished ZIKV and DENV-specific antibodies. Hence, this novel biosensor allows assessing ZIKV antibodies in blood and saliva and results are unaffected by presence of DENV virus-specific antibodies.

References

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Citations

Dec 28, 2019·International Journal of Molecular Sciences·Wei XuChung Chiun Liu
Jul 11, 2020·Scientific Reports·Alice F VersianiFlávio G da Fonseca
Oct 8, 2020·Mikrochimica Acta·Sebastian CajigasJahir Orozco
Aug 3, 2020·Biosensors & Bioelectronics·Tianxing JiQiang Zhou
Feb 10, 2021·Medicinal Research Reviews·Xianlong ZhangTony D James
Mar 2, 2019·Biosensors & Bioelectronics·Lei WangBrian J Geiss
Mar 12, 2021·Biosensors & Bioelectronics·K Yugender GoudK Vengatajalabathy Gobi
Jun 3, 2021·International Journal of Environmental Research and Public Health·Tomáš MackuľakAndrea Butor Škulcová
Jun 29, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alexander MacdonaldDamion K Corrigan
Jun 29, 2021·Trends in Analytical Chemistry : TRAC·Mazhar SherStefano Cinti
Aug 9, 2021·Talanta·Taís Aparecida Reis CordeiroLucas Franco Ferreira
Sep 24, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Samuel Santos PereiraLuís Carlos de Souza Ferreira
Mar 11, 2020·International Archives of Allergy and Immunology·Federico StorniMartin F Bachmann

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