Biosensor device for the photo-specific detection of immuno-captured bladder cancer cells using hexaminolevulinate: An ex-vivo study

Photodiagnosis and Photodynamic Therapy
Kit Man ChanMelanie MacGregor

Abstract

Exogenous administration of the photodynamic agent hexaminolevulinate induces Protoporphyrin IX (PpIX) accumulation in malignant tissue. This may enable differentiation from healthy tissues by emission of a distinctive red fluorescence. It provides the photo-specific detection when excited with blue light at 405 nm. This study determines the ex-vivo processing conditions (time, concentration, temperature and addition of a fluorescent dye) required for HAL-induced PpIX fluorescence to successfully discriminate between bladder cancer and benign fibroblast cells shed in urine at the single cell level. HAL-induced fluorescence was 4.5 times brighter in cancer cells than non-cancer cells when incubated in the optimum conditions, and could be used to correctly identified bladder cancer cells captured within a newly developed immunofunctionalized biosensor with 88% efficiency. This biosensor is designed to facilitate the immuno-capture of cancer cells by interaction with carcinoma specific anti Epithelial Cell Adhesion molecule (anti-EpCAM) antibodies. Anti-EpCAM antibodies were immobilized on polyoxazoline (POx) plasma polymers by covalent bonds in microfluidic channels. Combining photodynamic and immunoselective approach therefore c...Continue Reading

Citations

Jun 12, 2020·Diagnostics·Kit Man ChanMelanie MacGregor
May 18, 2020·Biointerphases·Hanieh Safizadeh ShiraziMelanie MacGregor
Apr 26, 2020·International Journal of Molecular Sciences·Kit Man ChanMelanie MacGregor
Apr 2, 2021·Scientific Reports·Kit Man ChanMelanie MacGregor
Oct 2, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Kola OstrikovMelanie N MacGregor

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