Biosensor for dengue virus detection: sensitive, rapid, and serotype specific

Analytical Chemistry
Antje J BaeumnerRichard A Montagna

Abstract

A serotype-specific RNA biosensor was developed for the rapid detection of Dengue virus (serotypes 1-4) in blood samples. After RNA amplification, the biosensor allows the rapid detection of Dengue virus RNA in only 15 min. In addition, the biosensor is portable, inexpensive, and very easy to use, making it an ideal detection system for point-of-care and field applications. The biosensor is coupled to the isothermal nucleic acid sequence-based amplification (NASBA) technique with which small amounts of virus RNA are amplified using a simple water bath. During the NASBA reaction, a generic sequence is attached to all RNA molecules as described earlier (Wu, S. J.; Lee, E. M.; Putvatana, R.; Shurtliff, R. N.; Porter, K R.; Suharyono, W.; Watt, D. M.; King, C. C.; Murphy, G. S.; Hayes, C. G.; Romano, J. W. J. Clin. Microbiol. 2001, 39, 2794-2798.). It has been shown earlier that Dengue virus can be detected specifically using two DNA probes: a first probe hybridized with the attached generic sequence and, therefore, bound to every amplified RNA molecule; and a second probe either bound to all four Dengue virus serotypes or chosen to be specific for only one serotype. These probes were utilized in the biosensor described in this pub...Continue Reading

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Citations

Sep 1, 2006·Analytical and Bioanalytical Chemistry·Katie A Edwards, Antje J Baeumner
Sep 6, 2006·Analytical and Bioanalytical Chemistry·Katie A Edwards, Antje J Baeumner
Mar 4, 2008·Analytical and Bioanalytical Chemistry·John T ConnellyAntje J Baeumner
Dec 5, 2008·Analytical and Bioanalytical Chemistry·Xuanhong ChengWilliam R Rodriguez
Sep 13, 2011·Analytical and Bioanalytical Chemistry·John T ConnellyAntje J Baeumner
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Jun 9, 2005·Journal of Virological Methods·Salin ChutinimitkulYong Poovorawan
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