DOI: 10.1101/479345Nov 26, 2018Paper

Biospeckle laser digital image processing for quantitative and statistical evaluation of the activity of Ciprofloxacin on Escherichia coli K-12

BioRxiv : the Preprint Server for Biology
Hilda Cristina GrassiEfrén D J Andrades

Abstract

Antibiotic susceptibility testing is a necessary step prior to the treatment of clinical infections. A major concern is the time required to obtain a fast and reliable result. The aim of this work is to use Biospeckle laser in a 15min assay for an antimicrobial susceptibility test of Ciprofloxacin in serial two-fold dilutions on Escherichia coli K-12 using Venereal Disease Research Laboratory (VDRL) plates. Analysis of images by video edition is performed on a quantitatively selected region of interest, and processed with ImageJ-ImageDP; and by the construction of time series and analysis with either statistical diagnostics tests or Autoregressive Integrated Moving Average (ARIMA) models. Antimicrobial susceptibility tests are also performed for the purpose of quantitative comparison, showing a profile that is comparable to the result obtained with ImageJ-ImageDP processing after 15min of antibiotic action. Only the time series of the least affected bacteria (low Ciprofloxacin concentration) behaves in an expected manner, being non-independent and mainly non-linear, non-normal, and heteroscedastic. The most affected bacteria (higher Ciprofloxacin concentration) are non-independent and tend to be linear, normal and heteroscedast...Continue Reading

Related Concepts

Antibiotics
Ciprofloxacin
Disease Susceptibility
Escherichia coli
Venereal Disease Research Laboratory
Antimicrobial Susceptibility Test (Procedure)
Evaluation
Statistical Test
Diagnostic Procedure
SECTM1 protein, human

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