Biostimulation of estuarine microbiota on substrate coated agar slides: a novel approach to study diversity of autochthonous Bdellovibrio- and like organisms

Microbial Ecology
Ashvini Chauhan, H N Williams

Abstract

Characterization of Bdellovibrio- and like organisms (BALOs) from environmental samples involves growing them in the presence of Gram-negative prey bacteria and isolation of BALO plaques. This labor-intensive enrichment and isolation procedure may impede the detection and phylogenetic characterization of uncultivable BALOs. In this article, we describe a simple slide biofilm assay to improve detection and characterization of BALO microbiota. Agar spiked with biostimulants such as yeast extract (YE), casamino acids (CA), or concentrated cells of Vibrio parahaemolyticus P5 (most widely used prey bacteria for isolation of halophilic BALOs) was plated onto buffed glass slides and exposed to water samples collected from Apalachicola Bay, Florida. After incubating for a week, diversity of the biofilm bacterial community was studied by culture-dependent and culture-independent molecular methods. The results revealed that most probable numbers (MPNs) of BALOs and total culturable bacteria recovered from YE agar slide were significantly higher than the numbers on CA- or P5-spiked agar slides. Polymerase chain reaction-restriction fragment length polymorphism followed by 16S rDNA sequencing of clones from different biostimulants resulted...Continue Reading

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Citations

Jul 7, 2009·Annual Review of Microbiology·R Elizabeth Sockett
Mar 31, 2012·PloS One·Huan ChenH N Williams
Feb 19, 2009·Research in Microbiology·Adrian A Medina, Daniel Kadouri
Dec 25, 2015·Microbiology and Molecular Biology Reviews : MMBR·Hongyue Dang, Charles R Lovell
Apr 8, 2021·Current Biology : CB·Benjamin R WucherCarey D Nadell

Related Concepts

Casamino acids
Agar
Bdellovibrio
DNA, Bacterial
Bionomics
Phylogeny
RNA, Ribosomal, 16S
Vibrio parahaemolyticus
Water Microbiology
Streak Plate Count

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