Biosurfactant production by free and alginate entrapped cells of Pseudomonas fluorescens.

Journal of Industrial Microbiology & Biotechnology
M AbouseoudRachida Maachi

Abstract

Production of biosurfactant by free and alginate-entrapped cells of Pseudomonas fluorescens Migula 1895-DSMZ was investigated using olive oil as the sole carbon and energy source. Biosurfactant synthesis was followed by measuring surface tension and emulsifying index E24 over 5 days at ambient temperature and at neutral pH. Diffusional limitations in alginate beads affected the kinetics of biosurfactant production when compared to that obtained with free cells culture. Nevertheless, the emulsion stability was improved and fewer by-products interfered with the biosurfactant activity. A decrease in pH down to 5 in the case of immobilized cells was observed during the first 3 days, after which it returned to its initial value. The minimum values of surface tension were 30 and 35 dynes cm(-1) achieved after 40 and 72 h with free and immobilized cells, respectively, while the corresponding maximum E24 values were 67 and 62%, respectively. After separation by acetone precipitation, the biosurfactant showed a rhamnolipid-type in nature, and had a good foaming and emulsifying activities. The critical micellar concentration was found to be 290 mg l(-1). The biosurfactant also showed good stability during exposure to high temperatures (u...Continue Reading

References

Jun 16, 2000·Applied Microbiology and Biotechnology·I M BanatS S Cameotra
May 23, 2002·Applied Biochemistry and Biotechnology·Alexandre S SantosDenise M G Freire
Mar 10, 2004·Biotechnology Letters·Hye-Sung JeongJai-Yul Kong
Aug 17, 2004·Applied Microbiology and Biotechnology·K-H ShinE A Seagren
Jan 22, 2005·Journal of Applied Microbiology·M BonillaM Soubes
Feb 1, 1987·Applied and Environmental Microbiology·D G Cooper, B G Goldenberg
Sep 1, 2006·Applied Biochemistry and Biotechnology·B MahantyV V Dasu

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Citations

Mar 26, 2010·Applied Microbiology and Biotechnology·Ahmad Mohammad Abdel-MawgoudEric Déziel
Apr 8, 2016·Environmental Science and Pollution Research International·Fatma Zohra MesbaiahMohamed Chamkha
Mar 18, 2015·Applied Microbiology and Biotechnology·Saúl AlonsoMario Díaz
May 29, 2009·FEMS Microbiology Letters·Alejandro P RooneyTsung-Min Kuo
Feb 26, 2011·Biotechnology Research International·Nilanjana Das, Preethy Chandran
Aug 2, 2018·Journal of Applied Microbiology·P SinghV Rale
Aug 7, 2017·Microbial Cell Factories·Huiqing Chong, Qingxin Li
Jul 25, 2017·ACS Synthetic Biology·Pei Kun R TayNeel S Joshi

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